中国组织工程研究Issue(38)：6069-6073,5.DOI:10.3969/j.issn.2095-4344.2014.38.001
双膦酸盐对破骨细胞分化及抗酒石酸酸性磷酸酶的影响
Effect of bisphosphonate on osteoclast differentiation and tartrate-resistant acid phosphatase
摘要
Abstract
BACKGROUND:Tartrate-resistant acid phosphatase is a specific marker for osteoclast differentiation and bone resorption, which is a sign of osteoclast maturity. <br> OBJECTIVE:To study the effect of alendronate on tartrate-resistant acid phosphatase related to osteoclast differentiation and bone resorption. <br> METHODOsteoclasts were cultured by mouse monocyte-macrophage cellline-RAW264.7. The cells were divided into two groupcontrol group, treated with 100μg/L receptor activator of nuclear factorκB ligand factor;alendronate group, treated with 100μg/L receptor activator of nuclear factorκB ligand factor+10-7 mol/L alendronate. Osteoclastogenesis and resorption function of osteoclasts were examined at 7 days of culture. Gene expression of tartrate-resistant acid phosphatase was detected by immunofluorescence method. Western blot assay was used to detect protein expression of tartrate-resistant acid phosphatase. <br> RESULTS AND CONCLUSION:Tartrate-resistant acid phosphatase positive multinuclear cells were observed and resorption lacunae formed in two groups. Control group showed the higher number of tartrate-resistant acid phosphatase positive multinuclear cells and larger size of resorption lacunae than the alendronate group (P<0.01). Immunofluorescence showed expression of tartrate-resistant acid phosphatase was higher in the control group than the alendronate group (P<0.01);furthermore, the protein expression of tartrate-resistant acid phosphatase was also lower in the alendronate group than the control group (P<0.01). These findings indicate that bisphosphonates could strongly inhibit osteoclastogenesis and its resorption function by inhibiting protein expression of tartrate-resistant acid phosphatase.关键词
组织工程/二膦酸盐类/破骨细胞/骨质疏松Key words
tissue engineering/diphosphonates/osteoclasts/osteoporosis分类
医药卫生引用本文复制引用
董伟,冯晓洁,梁永强,邓久鹏,温黎明,戚孟春..双膦酸盐对破骨细胞分化及抗酒石酸酸性磷酸酶的影响[J].中国组织工程研究,2014,(38):6069-6073,5.基金项目
国家自然科学基金(81270965)；河北省自然科学基金(C2011401044)Funding:the National Natural Science Foundation of China, No.81270965 （81270965）
the Hebei Natural Science Foundation, No. C2011401044 （）
