中国组织工程研究Issue(38)：6099-6109,11.DOI:10.3969/j.issn.2095-4344.2014.38.006
富血小板血浆协同骨形态发生蛋白4促进骨愈合的效应
Bone morphogenetic protein-4 compounded with platelet-rich plasma promotes bone healing
摘要
Abstract
BACKGROUND:Studies have shown that bone morphogenetic proteins play a key role in skeletal development. Platelet-rich plasma alone in animal or clinical trials cannot significantly promote bone graft healing. <br> OBJECTIVE:To investigate the osteogenesis effect of bone morphogenetic protein-4 compounded with platelet-rich plasma the bone defect area. <br> METHODTwenty-four New Zealand white rabbits were selected to establish maxil ary bone defect models, and then were randomly divided into four groups, six rats in each group. Group A was blank control group;Group B wasβ-tricalcium phosphate (0.1 g)+Bio-gide group;group C wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL) group;and group D wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL)+bone morphogenetic protein-4 (5μg). Gross observation, histological observation and imaging analysis were performed for analysis of new bone formation at weeks 4, 8, 12 after modeling. <br> RESULTS AND CONCLUSION:After 4 weeks, group D had more new bone and vessels formed in the bone defect area than the group B (P<0.01);however, the amount of new bone was higher in the group B than the group A (P<0.01). After 4, 8, 12 weeks, the amount of new bone in the bone defect area was higher in the group D than the groups B and C (P<0.01), and lowest in the group A (P<0.01). In theβ-tricalcium phosphate scaffold, platelet-rich plasma combined with bone morphogenetic protein can significantly promote the healing of bone defects.关键词
组织构建/骨组织工程/骨形态发生蛋白/富血小板血浆/颌骨/β-磷酸三钙Key words
maxil a/bone morphogenetic proteins/platelet-rich plasma/calcium phosphates分类
医药卫生引用本文复制引用
史剑杰,罗志宾,陈文雄..富血小板血浆协同骨形态发生蛋白4促进骨愈合的效应[J].中国组织工程研究,2014,(38):6099-6109,11.基金项目
广东省科技发展项目(2010B060900087)课题名称PRP协同BMP-4修复下颌骨缺损的动物实验研究Funding:the Science and Technology Development Program of Guangdong Province, No.2010B060900087 （2010B060900087）
