中国组织工程研究Issue(38)：6172-6178,7.DOI:10.3969/j.issn.2095-4344.2014.38.018
透明质酸对人骨关节炎软骨细胞骨桥蛋白和CD44表达的影响
Effects of hyaluronic acid on osteopontin mRNA and CD44 mRNA expression in human osteoarthritic chondrocytes
周斌 1张方杰 2罗伟 3高曙光 3曾超 3熊依林 3李宇晟 3雷光华2
作者信息
- 1. 中南大学湘雅医院，湖南省长沙市 410008
- 2. 中南大学湘雅医院 中南大学骨科研究所
- 3. 中南大学湘雅医院 骨科，湖南省长沙市 410008
- 折叠
摘要
Abstract
BACKGROUND:Progressive fracture of the cartilage is considered the characteristic lesion in later osteoarthritis, the expression of osteoarthritis-related factors such as hyaluronic acid, osteopontin and CD44 in osteoarthritic cartilage is increased. <br> OBJECTIVE:To investigate the effect of hyaluronic acid on the expression of osteopontin mRNA and CD44 mRNA of chondrocytes in the in vitro cultured chondrocytes of patients with knee osteoarthritis. <br> METHODThe cartilage samples obtained from osteoarthritic patients were cultured and purified into acquire chondrocytes in vitro, and the cells were divided into three groupblank control group, hyaluronic acid (100 mg/mL) group and hyaluronidase (200 mg/mL) group. After 48 hours of cellculture, real-time quantitative polymerase chain reaction assay was used to detect the expression of CD44 mRNA and osteopontin mRNA. The difference of the expression levels before and after the intervention of hyaluronic acid was compared and analyzed using SPSS 17.0 software. <br> RESULTS AND CONCLUSION:Compared with the blank control group, hyaluronic acid (100 mg/mL) upregulated osteopontin mRNA expression in the chondrocytes, hyaluronidase (200 mg/mL) also reduced osteopontin mRNA expression in the chondrocytes. The CD44 mRNA expression in the chondrocytes of hyaluronic acid (100 mg/mL) group and hyaluronidase (200 mg/mL) group was lower than that in the blank control group. Hyaluronic acid can upregulate the expression of the osteopontin mRNA expression in the osteoarthritic chondrocytes;the biphasic effects of hyaluronic acid on CD44 mRNA expression in osteoarthritic chondrocytes might be associated with the molecule weight of hyaluronic acid.关键词
组织构建/软骨细胞/透明质酸/骨关节炎/膝/实时荧光定量RT-PCR/骨桥蛋白/CD44Key words
osteoarthritis, knee/chondrocytes/osteopontin/hyaluronic acid/antigen, CD44分类
医药卫生引用本文复制引用
周斌,张方杰,罗伟,高曙光,曾超,熊依林,李宇晟,雷光华..透明质酸对人骨关节炎软骨细胞骨桥蛋白和CD44表达的影响[J].中国组织工程研究,2014,(38):6172-6178,7.
