中国组织工程研究Issue(41)：6567-6572,6.DOI:10.3969/j.issn.2095-4344.2014.41.002
纳米生物探针双标大鼠骨髓间充质干细胞的成活及生长
Survival and growth of nano-bioprobe double-labeled rat bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Superparamagnetic iron oxide (SPIO) labeling can trace the migration of stem cells in vivo, and the fluorescent DiI dye is suitable for marking and tracing cells because of its less influence on cellviability, proliferation and differentiation. <br> OBJECTIVE:To observe the effect and safety of SPIO and fluorescent DiI dye to double label bone marrow mesenchymal stem cells. <br> METHODS:The bilateral lower limbs of rats were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. Bone marrow mesenchymal stem cells were isolated by the whole bone marrow adherence method and purified by differential attachment method. Purified cells were dual-labeled with SPIO particle and fluorescent DiI dye. <br> RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells could be separated at 8-10 days after primary culture and the subculturing cycle was 3-4 days. Rat bone marrow mesenchymal stem cells could be effectively labeled with SPIO-DiI and the labeling efficiency was almost 100%. Blue irons contained in intracytoplasmatic vesicles could be observed clearly with Prussian blue staining, and the fluorescence microscopy showed red fluorescence at cytoplasm. Survival and apoptosis percentages obtained by MTT analysis were similar among labeled and unlabeled bone marrow mesenchymal stem cells that were both about 95%.These findings indicate that the rat bone marrow mesenchymal stem cells could be efficiently labeled with SPIO-DiI to construct a nano-bioprobe, without significant changes in morphology, viability and proliferation.关键词
干细胞/骨髓干细胞/纳米探针/骨髓间充质干细胞/细胞培养/超顺磁性氧化铁/DiIKey words
bone marrow/mesenchymal stem cells/cells, cultured/iron/carbocyanines分类
医药卫生引用本文复制引用
曹爱红,杨欣,郭子威,胡维..纳米生物探针双标大鼠骨髓间充质干细胞的成活及生长[J].中国组织工程研究,2014,(41):6567-6572,6.基金项目
江苏徐州市科技计划项目(XM12B043) （XM12B043）
