中国组织工程研究Issue(41)：6620-6627,8.DOI:10.3969/j.issn.2095-4344.2014.41.011
Wistar大鼠肌肉注射异种脐带间充质干细胞的安全性
Security of heterogeneous umbilical cord mesenchymal stem cells via intramuscular injection in Wistar rats
摘要
Abstract
BACKGROUND:So far, the short-term changes of various organs after injection of umbilical cord mesenchymal stem cells have been reported, but there are few studies on the long-term changes of various organs in healthy rats after repeated intramuscular injection of umbilical cord mesenchymal stem cells. OBJECTIVE:To observe the security of intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells. METHODS:Sixty male SPF Wistar rats were divided into six groups randomly:normal group (suspension liquid of umbilical cord mesenchymal stem cells);control group with culture solution;supernatant group (supernatant of human umbilical cord mesenchymal stem cells);low concentration group (0.25×105 human umbilical cord mesenchymal stem cells);moderate concentration group (1.0×105 human umbilical cord mesenchymal stem cells);high concentration group (4.0×105 human umbilical cord mesenchymal stem cells). Each rat was injected 0.8 mL liquid in muscle, 0.2 mL in each limb, twice at weeks 1 and 4. Biochemical tests were conducted before and after injection. At the end of 8 weeks, al the rats were kil ed and hematoxylin-eosin staining was done with the liver, spleen, lung, kidney, brain and muscle. <br> RESULTS AND CONCLUSION:There was no abnormal change about biochemical tests and hematoxylin-eosin staining after the intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells. No significant alteration was observed in the liver, spleen, lung, kidney, brain, and muscle of the limb after the injection of heterogeneous umbilical cord mesenchymal stem cells under suitable concentration. These findings indicate intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells at certain concentrations is safe and reliable.关键词
干细胞/脐带脐血干细胞/脐带间充质干细胞/肌肉注射/异种/安全Key words
umbilical cord/mesenchymal stem celltransplantation/injections, intramuscular/safety分类
医药卫生引用本文复制引用
张文祥,王思平,锡洪敏,李自普..Wistar大鼠肌肉注射异种脐带间充质干细胞的安全性[J].中国组织工程研究,2014,(41):6620-6627,8.基金项目
青岛市公共领域科技支撑计划项目 （）
