中国组织工程研究Issue(41)：6633-6638,6.DOI:10.3969/j.issn.2095-4344.2014.41.013
富血小板血浆及脐带间充质干细胞修复软骨损伤
Platelet-rich plasma plus human umbilical cord mesenchymal stem cells for cartilage repair
徐静 1王黎明 1周立冬 2吴美 2崔辉 2赵璟 2曾独娟 2张仲文 3刘爱兵2
作者信息
- 1. 安徽医科大学武警总医院临床学院，安徽省合肥市 230032
- 2. 武警总医院医学实验中心，北京市 100071
- 3. 武警总医院骨四科，北京市 100039
- 折叠
摘要
Abstract
BACKGROUND:Chondrocytes co-cultured with bone marrow stromal stem cells on the scaffold of platelet-rich plasma are found to proliferate, and besides proliferative growth, bone marrow stromal cells exhibit a tendency of differentiating into chondrocytes. OBJECTIVE:To study the effect of platelet-rich plasma and human umbilical cord mesenchymal stem cells (hUCMSCs) on cartilage repair. METHODS:Forty healthy New Zealand white rabbits were selected to establish models of cartilage defects, and then randomly divided into normal saline group, platelet-rich plasma group, hUCMSCs group and combination group. Platelet-rich plasma was prepared by using double centrifugations to prepare passage 3 hUCMSCs. After modeling, intra-articular injection of normal saline (0.5 mL), 12.5%platelet-rich plasma (0.5 mL), 1×107 hUCMSCs (0.5 mL), 12.5%platelet-rich plasma+1×107 hUCMSCs (total y 0.5 mL) was done in corresponding groups, respectively. After 12 weeks of modeling, the injured cartilage was grossly observed, and hematoxylin-eosin staining was used to observe cartilage repair under light microscope;according to the O'Driscol histologic standard, histological examination was performed. RESULTS AND CONCLUSION:The repair effect in the normal saline group was significantly better that in the platelet-rich plasma group, hUCMSCs group, combination group (P<0.05), while the platelet-rich plasma group and combination group also exhibit better outcomes than the hUCMSCs group (P<0.05). These findings indicate that both platelet-rich plasma and hUCMSCs can promote cartilage repair;moreover, platelet-rich plasma with or without hUCMSCs is superior to hUCMSCs alone in the cartilage repair.关键词
干细胞/脐带脐血干细胞/富血小板血浆/人脐带间充质干细胞/关节软骨/软骨损伤/骨关节炎Key words
platelet-rich plasma/mesenchymal stem cells/chondrocytes分类
医药卫生引用本文复制引用
徐静,王黎明,周立冬,吴美,崔辉,赵璟,曾独娟,张仲文,刘爱兵..富血小板血浆及脐带间充质干细胞修复软骨损伤[J].中国组织工程研究,2014,(41):6633-6638,6.
