中国组织工程研究Issue(41)：6639-6644,6.DOI:10.3969/j.issn.2095-4344.2014.41.014
口服肉苁蓉联合骨髓间充质干细胞移植治疗大鼠脊髓损伤
Cistanche deserticola plus bone marrow mesenchymal stem cell transplantation for treating spinal cord injury in rats
兰静 1闫金玉 1夏润福 1李剑锋1
作者信息
- 1. 内蒙古医科大学第二附属医院康复科，内蒙古自治区呼和浩特市 010030
- 折叠
摘要
Abstract
BACKGROUND:Studies have shown that bone marrow mesenchymal stem cells under certain conditions can differentiate into nerve cells. Bone marrow mesenchymal stem celltransplantation can rebuild nervous system function and improve functional disorders in patients. Glycosides of cistanche also have a protective effect against nerve cellinjury. Their combination has been less reported. OBJECTIVE:To observe the effectiveness of Cistanche deserticola and bone marrow mesenchymal stem celltransplantation on spinal cord injury in rats. METHODS:Fifty adult Wistar rats with spinal cord injury were randomly divided into four group:a Cistanche deserticola group (intragastric administration of 20 mL/kg Cistanche deserticola concentrated solution per day), a celltransplantation group (10μL of 1×108/L bone marrow mesenchymal stem cellsuspension), a combination group (10μL of 1×108/L bone marrow mesenchymal stem cellsuspension+intragastric administration of 20 mL/kg Cistanche deserticola concentrated solution per day) and a control group (intragastric administration of 20 mL/kg normal saline per day). The intragastric administration lasted for 30 days in each group. RESULTS AND CONCLUSION:After 30 days of treatment, the expression of Nestin was significantly higher in the combination group than the other groups. After 12 weeks, Basso, Beattie, and Bresnahan scores was significantly higher in the combination group than the other groups (P<0.05);somatosensory and motor evoked potential latencies were also improve significantly in the combination group compared with the other groups (P<0.05). These findings indicate that oral administration of Cistanche deserticola combined with bone marrow mesenchymal stem cells can significantly improve the motor and neurophysiological function of spinal cord injury rats. Cistanche deserticola can improve the survival of transplanted bone marrow mesenchymal stem cells in rats with spinal cord injury.关键词
干细胞/移植/肉苁蓉/骨髓间充质干细胞/脊髓损伤/Wistar大鼠Key words
bone marrow/mesenchymal stem cells/cistanche/spinal cord injuries/rats, Wistar分类
医药卫生引用本文复制引用
兰静,闫金玉,夏润福,李剑锋..口服肉苁蓉联合骨髓间充质干细胞移植治疗大鼠脊髓损伤[J].中国组织工程研究,2014,(41):6639-6644,6.
