中国组织工程研究Issue(41)：6649-6654,6.DOI:10.3969/j.issn.2095-4344.2014.41.016
不同剂量骨碎补对兔骨髓间充质干细胞增殖分化的影响
Drynaria freeze-dried powder at different dosages influences proliferation and differentiation of rabbit bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Modern research shows that Drynaria can delay celldegeneration and reduce the incidence of osteoarthritis. OBJECTIVE:To observe the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells induced by different dosages of Drynaria freeze-dried powder, and to explore the optimum induction concentration. METHODS:Rabbit bone marrow mesenchymal stem cells were isolated and cultured in vitro by density gradient centrifugation and adherence screening methods, and then divided into blank group, positive control group (transforming growth factorβ1), high-, middle-, low-dosage Drynaria groups (0.4 mg, 0.1 mg, 5μg). Passage 3 cells were selected and cultured in different media. After 1 week, cellviability was detected by MTT method, and expression of type II col agen by immunohistochemical method. RESULTS AND CONCLUSION:Both transforming growth factorβ1 and Drynaria could improve the proliferation of bone marrow mesenchymal stem cells, and the increase in cellproliferation was ranked as fol ows:positive control group>low-dosage group>middle-dosage group>high-dosage group>blank group. Bone marrow mesenchymal stem cells were differentiated into chondrocytes under induction of transforming growth factorβ1 and Drynaria, and induced cells significantly expressed type II col agen. The expression of type II col agen was ranked as fol ows:positive control group>low-dosage group>middle-dosage group>high-dosage group>blank group. These findings suggest that Drynaria can promote the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells, and the optimal dosage is 5μg.关键词
干细胞/骨髓干细胞/骨碎补/骨髓间充质干细胞/增殖/诱导分化/组织工程学/兔Key words
bone marrow/mesenchymal stem cells/polypodiaceae/cellproliferation分类
医药卫生引用本文复制引用
张虎林,张自强,许宏斌,张晓刚..不同剂量骨碎补对兔骨髓间充质干细胞增殖分化的影响[J].中国组织工程研究,2014,(41):6649-6654,6.基金项目
甘肃省科技支撑计划-社会发展计划项目(0804NKCA101) （0804NKCA101）
