中国组织工程研究Issue(41)：6671-6677,7.DOI:10.3969/j.issn.2095-4344.2014.41.020
肌肉注射人脐带间充质干细胞健康大鼠心肌组织ki-67、phh3及cTnT的表达
Effects of human umbilical cord mesenchymal stem cells via intramuscular injection on the myocardial ki-67, phh3 and cTnT expression in normal rats
摘要
Abstract
BACKGROUND:It is unclear whether intramuscular injection of human umbilical cord mesenchymal stem cells wil increase regeneration of normal myocardial cells and play adverse effects on normal myocardium. OBJECTIVE:To observe the effects of intramuscular injection of human umbilical cord mesenchymal stem cells on expression of the myocardial ki-67, phh3 and cTnT in normal Wistar rats. METHODS:A total of 60 male Wistar rats were divided into six groups randomly:normal group, solution group, supernatant group, low concentration group, moderate concentration group, and high concentration group. These groups were intramuscularly injected different liquid, respectively, as fol ows:PBS, DMEM, the supernatant of human umbilical cord mesenchymal stem cells, human umbilical cord mesenchymal stem cells with amount of 0.25×105, human umbilical cord mesenchymal stem cells with amount of 1.0×105, human umbilical cord mesenchymal stem cells with amount of 4.0×105 . After 4 weeks, each rats received the second same intramuscular injections of human umbilical cord mesenchymal stem cells. Four weeks after the second injection, al rates were kil ed to obtain myocardial tissues which were fixed, embedded and sectioned. Final y, the ki-67, phh3 and cTnT expressions of the myocardium were detected by immunohistochemical technique. RESULTS AND CONCLUSION:In the normal group, negative expression of the ki-67 was observed in the caryon of myocardial cells, weak positive expression of the phh3 was observed in the caryon of myocardial cells, and positive expression of the cTnT was observed in the caryon of myocardial cells. Compared with the normal group, the expression of the ki-67, phh3 and cTnT in the myocardium had no significant difference among the other groups (F=1.076, 0.167, 0.300;P>0.05). Human umbilical cord mesenchymal stem cells or the supernatant of human umbilical cord mesenchymal stem cells via intramuscular injection have no effects on the expression of the ki-67, phh3 and cTnT in normal Wistar rats.关键词
干细胞/脐带脐血干细胞/脐带间充质干细胞/肌肉注射/ki-67/phh-3/cTnT/心肌组织Key words
umbilical cord/mesenchymal stem cells/injections, intramuscular/myocardium/troponin T/Ki-67 antigen分类
医药卫生引用本文复制引用
王琪,张文祥,王思平,李自普..肌肉注射人脐带间充质干细胞健康大鼠心肌组织ki-67、phh3及cTnT的表达[J].中国组织工程研究,2014,(41):6671-6677,7.基金项目
青岛市公共领域科技支撑计划项目[11-2-3-1-(4)-nsh] （4）
