中国组织工程研究Issue(42)：6769-6774,6.DOI:10.3969/j.issn.2095-4344.2014.42.009
人源核不均一核糖核蛋白E1真核表达载体在神经细胞中的表达
Expression of recombinant plasmid of homo heterogeneous nuclear ribonucleoprotein E1 in SH-SY5Y cells
摘要
Abstract
BACKGROUND:The functions of homo heterogeneous ribonucleoprotein E1 are very wide. It can participate in the expression of skeleton proteins in the nervous system. OBJECTIVE:To construct the recombinant plasmid of homo heterogeneous ribonucleoprotein E1 and observe its expression in nerve cells for further studying the functions of it in neurocytes. METHODS:Using pcDNATM4/His C, the homo heterogeneous ribonucleoprotein E1 was subcloned into recombinant plasmid E1-pcDNATM 4/His C, fol owed by enzyming and sequencing. After SH-SY5Y cells were transfected with the recombinant plasmid, western blot analysis and real time RT-PCR were used to detect the expression of homo heterogeneous ribonucleoprotein E1 in SH-SY5Y cells. And the growth of SH-SY5Y cells was observed. RESULTS AND CONCLUSION:We successful y constructed the eukaryotic expressed vector of homo heterogeneous ribonucleoprotein E1. The recombinant plasmids were verified to express in SH-SY5Y cells correctly at mRNA and protein levels. And SH-SY5Y cells generated quickly after homo heterogeneous ribonucleoprotein E1 was over-expressed. The homo heterogeneous ribonucleoprotein E1 is an important protein in neural development. And this vector offers the premise for further studying its function in nervous system.关键词
组织构建/组织工程/核不均一核糖核蛋白E1/表达载体/神经细胞/国家自然科学基金Key words
ribonucleoprotein, heterogeneous/neurons/transfection分类
医药卫生引用本文复制引用
霍丽蓉,王兰英,邹俊华,钟南..人源核不均一核糖核蛋白E1真核表达载体在神经细胞中的表达[J].中国组织工程研究,2014,(42):6769-6774,6.基金项目
国家自然科学基金(31200811)；北京市卫生系统高层次卫生技术人才培养计划“学科骨干”(2013-3-096) （31200811）
