中国组织工程研究Issue(45)：7294-7298,5.DOI:10.3969/j.issn.2095-4344.2014.45.015
超声微泡增效移植间充质干细胞的归巢机制
Mechanism of ultrasound-mediated microbubbles enhancing homing effect of transplanted mesenchymal stem cells
摘要
Abstract
BACKGROUND:Animal studies have indicated ultrasound-mediated microbubbles can significantly enhance the effect of stem cel transplantation to treat ischemic diseases. But its mechanism is stil unknown. <br> OBJECTIVE:To explore the mechanism of ultrasound-mediated microbubbles to significantly enhance the effect of stem cel transplantation in the treatment of ischemic diseases. <br> METHODS:Bone marrow mesenchymal stem cel s and vascular endothelial cel s of rats were cultured in vitro, and then randomized to three groups:control group with no intervention, ultrasound group exposed to ultrasound at 1 MHz, 1 W/cm2 for 90 seconds, and ultrasound-mediated microbubble group treated with 5μL liposomes ultrasound microbubbles containing fluorocarbon gases (about 2×1011/L) and ultrasound exposure at 1 MHz, 1 W/cm2 for 90 seconds. <br> RESULTS AND CONCLUSION:Compared to the control group, ultrasound-mediated microbubbles significantly increased expressions of vascular endothelial growth factor and stromal cel-derived factor 1 in the supernatant of <br> vascular endothelial cel s (P<0.05);ultrasound had no effect on the expression of vascular endothelial growth factor, but decreased the level of stromal cel-derived factor 1 (P<0.01). Ultrasound-mediated microbubbles and the ultrasound alone could significantly enhance the CXCR4 gene expression in bone marrow mesenchymal stem cel s as compared with the control group (P<0.01), but there was no difference between the ultrasound-mediated microbubble group and the ultrasound group (P>0.05). These findings suggest that 1 W/cm2 ultrasound-mediated microbubbles can promote vascular endothelial growth factor and stromal cel-derived factor 1 secretion by vascular endothelia cel s, and meanwhile promote CXCR4 gene expression in bone marrow mesenchymal stem cel s. This may be the mechanism of the ultrasound-mediated microbubbles enhancing homing effect of transplanted stem cel s.关键词
干细胞/移植/超声/超声微泡/血管内皮细胞/间充质干细胞/基质细胞衍生因子1/血管内皮生长因子/干细胞归巢/心肌梗死Key words
sonication/endothelial cel s/mesenchymal stem cel s/liposomes分类
医药卫生引用本文复制引用
钱健,陈飞,范国峰,沙杜鹃,王路娜,李启明,马浩,陈一冰,张均..超声微泡增效移植间充质干细胞的归巢机制[J].中国组织工程研究,2014,(45):7294-7298,5.基金项目
江苏省卫生厅青年科研课题(Q201306)；南京市医学科技发展项目基金(YKK12071)；上海市闵行区科技局自然科学研究课题(2014MHZ037)@@@@Funding:Youth Scientific Research Project of Jiangsu Provincial Health Department, No. Q201306 （Q201306）
Nanjing Medical Technology Development Project Funds, No. YKK12071 （）
Natural Science Research Project of the Science and Technology Bureau of Minhang District in Shanghai, No.2014MHZ037 （）
