中国组织工程研究Issue(45)：7333-7336,4.DOI:10.3969/j.issn.2095-4344.2014.45.021
干细胞源性血管依从性与缺血态组织二次血流的重建
Compliance of ischemic tissue to stem cells-derived vessels for secondary revascularization
摘要
Abstract
BACKGROUND:To explore the methods for secondary limb revascularization after endovascular repair is one of the most urgent research topics. <br> OBJECTIVE:To investigate the biological mechanism about the transplantation of autologous peripheral blood stem cel s to lead and perfect the effect of ischemic tissue for secondary revascularization. <br> METHODS:Forty-two patients with critical low limb ischemia were selected and treated with endovascular repair as first revascularization and transplantation of autologous peripheral blood stem cel s as secondary revascularization. The secondary revascularization was carried out at 3 months after the first blood flow remodeling. The patients receiving secondary revascularization were fol owed for 4 years. <br> RESULTS AND CONCLUSION:After transplantation of autologous peripheral blood stem cel s, the patients complained that the rest pain of the lower leg was relieved obviously and intermittent claudication distance was significantly lengthened. Limb salvage rate was 100%. Skin temperature index was 1.6±0.3, transcutaneous oxygen pressure was (5.00±1.26) kPa, ankle-brachium index was 0.9±0.2, photoplethysmograpy index was 0.8±0.1, saturation of blood oxygen was (79.4±20.4)%, and digital subtraction angiography score was (1.3±0.2) <br> points. After transplantation, local blood flow indicators of the low limbs were positively correlated to local symptom indicators of the limbs (0.6<r<0.7, P<0.05). These results strongly suggest that the stem cel s-derived neovascularization can offer effective and permanent blood flow perfusions to the ischemic tissue, and this biological effect play an important role in secondary revascularization of the ischemic tissue.关键词
干细胞/移植/下肢动脉缺血/干细胞临床应用/自体外周血干细胞/血流重建/血管新生/云南省自然科学基金Key words
lower extremity/ischemia/angioplasty/peripheral blood stem cel transplantation/neovascularization, physiologic分类
医药卫生引用本文复制引用
何晓明,杨镛,万嘉..干细胞源性血管依从性与缺血态组织二次血流的重建[J].中国组织工程研究,2014,(45):7333-7336,4.基金项目
云南省自然科学基金(2008CD205，2010ZC219)；云南省学术与技术带头人后备人才基金(2008PY004)@@@@Funding:the Natural Science Foundation of Yunnan Province, No.2008CD205,2010ZC219 （2008CD205，2010ZC219）
the Middle and Young Aged Academic and Technology Leaders Reserve Personnel Foundation of Yunnan Province, No.2008PY004Funding:the Natural Science Foundation of Yunnan Province, No.2008CD205,2010ZC219 （）
the Middle and Young Aged Academic and Technology Leaders Reserve Personnel Foundation of Yunnan Province, No.2008PY004 （）
