中国组织工程研究Issue(49)：7974-7978,5.DOI:10.3969/j.issn.2095-4344.2014.49.018
构建大鼠减体积肝移植模型的肝脏信号转导差异蛋白表达
Expression of hepatic signal transduction proteins following reduced-size liver transplantation in rats
刘静 1李立 1冉江华 1张升宁 1李来邦 1高扬 1陈奕明 1张熙冰1
作者信息
- 1. 昆明医科大学附属甘美医院 昆明市第一人民医院肝胆胰外科，云南省昆明市 650011
- 折叠
摘要
Abstract
BACKGROUND:The proteome is a highlight technology in medical research fields lately, and has been reported to be applied in basic research fields related to liver transplantation. However, it has not been heard that the proteome has been used in research related to reduced-size liver transplantation. OBJECTIVE: To study expression of hepatic differential proteins related to signal transduction using proteomics after reduced-size liver transplantation in rats. METHODS:On the basis of successful establishment of rat models of reduced-size liver transplantation, transplanted liver tissues were obtained at 1, 3 and 7 days after transplantation. Postoperative liver tissue and normal donor, receptor liver tissues were subjected to solid pH gradient two-dimensional gel electrophoresis. Two-dimensional gel electrophoresis patterns were set up. Differentialy expressed protein spots were identified using tandem mass spectrometry analysis and database. RESULTS AND CONCLUSION:Seventy-two differential protein stains were found taking 10 times measure. Finaly, 32 proteins with clear functions were identified. Of them, four proteins participated in signal transduction, and they distributed at 3 and 7 days after liver transplantation, accounting for 6%. Results verified that on the basis of successful and stable establishment of rat models of reduced-size liver transplantation, proteomics technology was utilized to study differential proteins involving in signal transduction after reduced-size liver transplantation, and this study provides data for further deep investigation of regulating MicroRNA of these proteins.关键词
实验动物/组织工程/减体积肝移植/大鼠/差异蛋白/信号转导Key words
liver transplantation/adaptor proteins, signal transducing/rats分类
医药卫生引用本文复制引用
刘静,李立,冉江华,张升宁,李来邦,高扬,陈奕明,张熙冰..构建大鼠减体积肝移植模型的肝脏信号转导差异蛋白表达[J].中国组织工程研究,2014,(49):7974-7978,5.
