中国组织工程研究Issue(25)：4048-4053,6.DOI:10.3969/j.issn.2095-4344.2015.25.022
筋膜成纤维细胞与纤维蛋白凝胶的体外生物相容性
Biocompatibility in vitro between fascia fibroblasts and fibrin glue
摘要
Abstract
BACKGROUND:Fibrin glue is a natural biodegradable scaffold, which can be used for tissue-engineered scaffolds, and is increasingly used as seed cel carrier for tissue engineering repair. OBJECTIVE:To investigate the biocompatibility in vitro of rabbit fascia fibroblasts and fibrin glue. METHODS:Tissue explants adherent method was used to culture fibroblasts from subcutaneous deep fascia tissue of New Zealand white rabbits. The fibroblasts could be passaged with trypsin digestion method. Suspension of passage four fibroblasts was co-cultured with fibrin glue. Morphology and proliferation of fibroblasts on the surface of fibrin glue were dynamical y observed under the inverted phase contrast microscope. At 5 days after co-culture, fibroblasts were identified by immunofluorescence staining under the laser scanning confocal microscope. The fibroblast growth and adhesion were observed under the scanning electron microscope. RESULTS AND CONCLUSION:There was no significant difference in fibroblast morphology between co-culture fibroblasts and pure culture fibroblasts with inverted phase contrast microscope. Scanning electron microscope demonstrated that fibroblasts ful y extended in fibrin glue surface, and showed a good adhesion between the“pseudopodium”and fibrin glue, and secreted matrix material. It is clear that the fibrin glue did not alter the morphologic features of fibroblasts. Laser scanning confocal microscope revealed that fibroblasts were positive for vimentin. These verified that properties of fibroblasts did not change after they were seeded in fibrin glue surface and did not be induced to differentiate. There is a very good biocompatibility between fascia fibroblasts and fibrin glue in vitro.关键词
生物材料/材料相容性/纤维蛋白凝胶/成纤维细胞/生物相容性/兔/筋膜/国家自然科学基金Key words
Tissue Engineering/Fascia/Fibrin/Fibroblasts分类
医药卫生引用本文复制引用
辛培成,郭其勇,李杰,赵德伟,杨圣..筋膜成纤维细胞与纤维蛋白凝胶的体外生物相容性[J].中国组织工程研究,2015,(25):4048-4053,6.基金项目
国家自然科学基金资助项目(30870647-C100202) （30870647-C100202）
