中国组织工程研究Issue(27)：4361-4364,4.DOI:10.3969/j.issn.2095-4344.2015.27.018
以改良方法建立兔VX2肝癌模型
Improved methods of establishing rabbit VX2 hepatocarcinoma models
摘要
Abstract
BACKGROUND:Rabbit VX2 hepatocarcinoma models are characterized by expanded and infiltrative growth, abundant blood supply, similar pathological and imaging features to human hepatocelular carcinoma, so this model is ideal for imaging experiments. OBJECTIVE: To establish rabbit VX2 hepatocarcinoma models and compare two different laparotomy methods in establishing rabbit VX2 hepatocarcinoma models. METHODS:Thirty New Zealand rabbits were randomly divided into two groups: traditional laparotomy (tumor particles were directly embedded into the liver with tweezers) and improved laparotomy (tumor particles were injected into the liver through a 15G syringe needle). The growth features of the tumor established in two different ways were compared by ultrasound. RESULTS AND CONCLUSION:The tumor formation rate was 100% in the two groups; the single rate was respectively 50% and 90%; the ectopic implantation rate was 50% and 10%, there were significant differences between two groups (P < 0.05). The mortality rate within 2 weeks was 33% and 0 in the two groups, with significant differences (P < 0.05). The improved laparotomy method is feasible for modeling rabbit VX2 hepatocarcinoma, due to minimal injury, short operation time, high success rate and stable features.关键词
实验动物/消化系统损伤动物模型/兔/VX2 肿瘤/肿瘤模型/移植方式/瘤组织块接种/实验性/超声/动物模型/肝癌Key words
Neoplasms/Models, Animal/Transplantation/Ultrasonography分类
医药卫生引用本文复制引用
童敏,陈柱..以改良方法建立兔VX2肝癌模型[J].中国组织工程研究,2015,(27):4361-4364,4.基金项目
Funding:grants from General Program of Hunan Provincial Science and Technology Bureau, No.2014TT2017湖南省科学技术厅科技计划一般项目(2014TT2017) （2014TT2017）
