摘要
Abstract
BACKGROUND:Several studies have demonstrated that the physiological process of the ovary, including folicle development, maturity, ovulation, corpus luteum formation and regression, is related to the formation of extracelular matrix. However, matrix metaloproteinase 9 and connective tissue growth factors had a close relationship with the formation of extracelular matrix. OBJECTIVE:To investigate the relationship between the expression of connective tissue growth factor and matrix metaloproteinase 9 and polycystic ovary syndrome. METHODS:The female SD rats with regular estrous cycle were randomly divided into control and model groups. The rats in the model group were intragastricaly with letrozole to establish the model of polycystic ovary syndrome, while the rats in the control group were intragastricaly with the same amount of carboxymethyl celulose. When the natural law of estrous cycle of rats in the model group did not exist and consecutive interval appeared, rat serum and ovarian tissues were colected. Rats in the control group were detected by colecting specimen in the interval. RESULTS AND CONCLUSION:Radioimmunoassay and immunohistochemical staining results showed that compared with the control group, the expression of serum luteinizing hormone, serum prolactin levels, connective tissue growth factor in preantral folicles theca cytoplasm, connective tissue growth factors in folicular basement membrane cytoplasm, connective tissue growth factors in albuginea and matrix metaloproteinase 9 in corpus luteum significantly increased (P < 0.05). The contents of folicle stimulating hormone and serum estradiol, and expression of matrix metaloproteinase 9 in folicular basement membrane significantly decreased (P < 0.05). The results indicate that connective tissue growth factors may be related to the excessive growth of smal folicles and ovulation disorders in polycystic ovary syndrome, while matrix metaloproteinase 9 may be related to ovulation disorders in polycystic ovary syndrome.关键词
实验动物/基因病毒载体及相关因子动物模型/多囊卵巢综合征/结缔组织生长因子/基质金属蛋白酶9/细胞外基质/促黄体生成激素/卵泡基底膜/大鼠多囊卵巢综合征模型/排卵障碍/卵巢局部调节因子/DAB显色Key words
Tissue Engineering/Ovary/Connective Tissue Growth Factor/Matrix Metaloproteinase 9/Extracelular Matrix分类
医药卫生
