现代医院Issue(8)：11-13,3.DOI:10.3969/j.issn.1671-332X.2014.08.004
异烟肼敏感与耐药结核杆菌临床分离株培养滤液蛋白比较分析
COMPARATIVE ANALYSIS OF CULTURE FILTRATE PROTEINS BETWEEN ISOLATED MY-COBACTERIUM TUBERCULOSIS STRAIN RESISTANTS AND Sensitivity TO ISONIAZID
摘要
Abstract
Objective To find the specific secretory proteins of Mycobacterium tuberculosis resistant to isoniazid. Methods The 7-day and 14-day cultures filtrates of a clinical isolate of isoniazid-resistant Mycobacterium tuberculosis and a clinical isolate of isoniazid-sensitive Mycobacterium tuberculosis growing in Middlebrook 7H9 were captured by weak cation exchange protein chip (WCX2) and detected by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).Differences were analyzed with descriptive statistics method.Results One hundred sixty four and one hundred seventy five proteins existed in the 7-day and 14-day cultures filtrates of isoniazid-sensitive Myco-bacterium tuberculosis, while 133 and 147 proteins were detected respectively for clinical isolate of isoniazid-resistant Myco-bacterium tuberculosis.Depending on the ratio of mass, their relative molecular masses to charge were 982 ~9328, 997 ~9327, 1050~8903 and 996 ~9369 and the abundance represented relative intensity value calculated by mass spectrometry peak area were 17~2100,21~1943,60~5355 and 41~3267 respectively.Compared with isoniazid-sensitive Mycobacteri-um tuberculosis strain, there were 47 and 58 different proteins in the 7-day and 14-day cultures filtrates of isoniazid-re-sistant Mycobacterium tuberculosis strain.Their relative molecular masses range from 1 083 to 8 812 and from 1 012 to 9327, and levels of abundance were in the range of 76 to 1 811 and 25 to 3 010.Conclusion There are differences of proteins in the culture filtrate during the early and middle of culture courses between isoniazid-resistant and isoniazid-sensitive Myco-bacterium tuberculosis strain.But it remains to be further confirmed whether these different proteins come from secretory pro-tein of Mycobacterium tuberculosis or not.关键词
结核杆菌/培养滤液蛋白/异烟肼/表面增强激光解析-电离飞行时间质谱( SELDI-TOF-MS)Key words
Mycobacterium tuberculosis/Culture filtrate protein/Isoniazid/Surface-enhanced laser desorption/i-onization time-of-flight mass spectrometry ( SELDI-TOF-MS)引用本文复制引用
曹翌明,刘志辉,孟繁荣,谢贝,蔡杏珊,黄业伦,牛群,雷杰,高俊文,谭耀驹..异烟肼敏感与耐药结核杆菌临床分离株培养滤液蛋白比较分析[J].现代医院,2014,(8):11-13,3.基金项目
国家“十二五”传染病防治重大科技专项（编号2012ZX10004-903）；广东省科技计划项目 （）
