现代医院Issue(1)：13-15,3.DOI:10.3969/j.issn.1671-332X.2015.01.003
永生化骨髓间充质干细胞模型的构建
CONSTRUCTION OF IMMORTALIZEDBONE MARROW MESENCHYMAL STEM CELLS
程斌 1程海燕2
作者信息
- 1. 广州中医药大学祈福医院 广东广州 511495
- 2. 南昌大学第二附属医院 江西南昌 330000
- 折叠
摘要
Abstract
Objective To establish immortalized bone marrow mesenchymal stem cells (BMSCs) from SD rats in vitro for further research on the characteristics and clinical application of BMSCs.Methods By using L ipo-fectamine TM 2000 -mediated gene transfection, plasmid pCMVSV40T/PUR containing the simian virus 40 large T antigene gene (SV40Tag) was transfected into BMSCs.BMSCs were screened by puromycin and then cultured on an extended scale.Their cell morphology and growth conditions were observed.Growth curve of cells was graphed.The expression of SV40Tag in transfected cells was identified by enzyme digestion method for the detection of tumor forma-tion.Results The growth rate of the experimental group of BMSCs after the 7th generation was significantly higher than that of the control group.No tumorigenicity was found in BMSCs after the 7th generation.Conclusion In vitro, the immortalized BMSCs by pCMVSV40T/PUR can provide basis on a large scale for its application in clinic and sci-entific research.关键词
SV40T/骨髓间充质干细胞/永生化/构建/模型Key words
SV40T/Bone marrow mesenchymal stem cells (BMSCs)/Immortalized/Construction/Model引用本文复制引用
程斌,程海燕..永生化骨髓间充质干细胞模型的构建[J].现代医院,2015,(1):13-15,3.
