现代医药卫生Issue(17)：2568-2570,3.DOI:10.3969/j.issn.1009-5519.2014.17.004
三丁基锡对蛋白磷酸酶2A的抑制机制
Inhibition mechanism of TBT to PP2A activity in human amnionic cells
摘要
Abstract
Objective To investigate the inhibition mechanism of tributyltin(TBT) on protein phosphatase 2A(PP2A) activity in human amnionic FL cells. Methods 1 h after contamination by different concentrations of TBT,FL cells were detected on the PP2A activity,levels of PP2A subunits including A,C,B55αand B56δ,protein phosphorylation of C and methylation level. Results Compared with control group,the activity of PP2A in FL cells was declined when the concentration was in 3,4 and 6μmol/L in TBT groups with statistically significant difference(P<0.01);PP2A-A subunit was decreased in 6μmol/L TBT group, and B55αsubunit and the methylation of PP2A-C were significantly decreased in the 4μmol/L and 6μmol/L TBT groups,the dif ferences all had statistically significant differnce(P<0.05). Conclusion The inhibitory effect of IBT to PP2A in FL cells may be associated with modifications of B55αand A subunit and reduction of PP2A-C methylation in Leu309 area.关键词
锡/蛋白磷酸酶2/羊膜/抑制因子,免疫/全酶Key words
Tin/Protein phosphatase 2/Amnion/Suppressor factors,immunologic/Holoenzymes引用本文复制引用
张亚莉,孙利军..三丁基锡对蛋白磷酸酶2A的抑制机制[J].现代医药卫生,2014,(17):2568-2570,3.基金项目
江苏省自然科学青年基金（BK20130397，BK20130389）；南通大学博士引进人才启动基金（13R26）。 （）
