现代医药卫生Issue(19)：2881-2883,2886,4.DOI:10.3969/j.issn.1009-5519.2014.19.001
三苯氧胺对肺癌A549/DDP细胞多药耐药性的逆转作用及机制
Effects and Mechanisms of Tamoxifen on Reversing the Multidrug Resistance of Lung Cancer A549/DDP Cells
李爱华 1宋丽华2
作者信息
- 1. 山东省兖矿集团公司总医院呼吸内科，山东邹城273500
- 2. 山东省医学科学院/济南大学医学与生命科学学院，山东济南250022
- 折叠
摘要
Abstract
Objective To explore the reversal effect of Tamoxifen(TAM) on multidrug resistance(MDR) in lung cancer A549/DDP cells and its proposed mechanisms. Methods The growth inhibitory rate of TAM in A549/DDP cells was tested by MTT colorimetry,and the effect of TAM on Rho123 in the A549/DDP cells was detected by flow cytometry,and then,the appro-priate experimental dose of TAM was determined. The MTT colorimetry was employed to detect the reversion efficiency of TAM on the AMD,GEM and DDP,namely,the real-time PCR colorimetry was applied to detect the changes of TAM on the multidrug-re-sistant gene MDR1 mRNA level of A549/DDP cells and the Western blotting assay to test the changes of the expression level of the MDR1 gene product P-gp. Results TAM at the dose of 10μmol/L had little cytotoxicity on A549/DDP cells and could in-crease the storage of Rho123 in the cells,as well as improve the insensitivity of chemotherapeutics,while the reversal multipliers to ADM,GEM and DDP are 3.0,7.2 and 2.0 respectively. The expression rate of MDR1 mRNA was reduced by 35%. It could in-hibit significantly the expression level of MDR1 gene product P-gp. Conclusion TAM can reverse the MDR of A549/DDP cells externally and improve its drug sensitivities,which is probably associated with inhibiting MDR1 gene expression and decreasing the intracellular ADR accumulation.关键词
药物耐受性/癌,非小细胞肺/转染/三苯氧胺/A549/DDP细胞Key words
Drug tolerance/Carcinoma,Non-Small-Cell Lung/Transfection/Tamoxifen/A549/DDP cells引用本文复制引用
李爱华,宋丽华..三苯氧胺对肺癌A549/DDP细胞多药耐药性的逆转作用及机制[J].现代医药卫生,2014,(19):2881-2883,2886,4.
