现代医药卫生Issue(21)：3206-3208,3.DOI:10.3969/j.issn.1009-5519.2014.21.003
地塞米松诱导人肺腺癌A549细胞对紫杉醇耐药性及Bcl-xL基因表达的影响
Influence of lung adenocarcinoma A549 cells induced by dexamethasone on the drug resistance of PTX and expression of Bcl-xL gene
摘要
Abstract
Objective To observe the changes of the drug resistance of lung adenocarcinoma A549 cells and the expres-sion of mRNA and protein of Bcl-xL in human lung adenocarcinoma A549 cell after dexamethasone(DEX) pretreatment in dif-ferent concentrations and intervention with paclitaxel(PTX),and to explore the molecular mechanism of DEX-induced A549 cell to the drug resistance of PTX. Methods The cell viability rate of lung adenocarcinoma A549 cells was determined by MTT assay after treatment of different concentrations of PTX,and to screen the half inhibitory concentration(IC50) of PTX. The cell viability rate of A549 cells which pretreated with different concentrations of DEX and different concentrations of PTX was determined by MTT assay,and the expression level of mRNA and protein of Bcl-xL in the A549 cells were determined by reverse transcriptase-poly merase chain reaction(RT-PCR) and Western blotting. Results After being pretreated with DEX in different concentrations, A549 cells were induced to resist to PTX,and the rate of resistance increased gradually with the increasing concentrations of DEX and the expression level of Bcl-xL gene and protein also increased gradually with the increasing of DEX concentrations. Conclu-sions DEX can induce resistance to PTX in lung adenocarcinoma A549 cells ,whose mechanism might be involved in increase of Bcl-xL gene(anti-apoptosis gene) in DEX-induced lung adenocarcinoma A549 cells.关键词
地塞米松/肺肿瘤/腺癌/紫杉酚/抗药性/基因表达/细胞凋亡Key words
Dexamethasone/Lung neoplasms/Adenocarcinoma/Paclitaxel/Drug resistance/Gene expres-sion/Apoptosis引用本文复制引用
康马飞,李林凤..地塞米松诱导人肺腺癌A549细胞对紫杉醇耐药性及Bcl-xL基因表达的影响[J].现代医药卫生,2014,(21):3206-3208,3.基金项目
广西壮族自治区卫生厅计划项目（Z2013479）。 （）
