现代医药卫生Issue(21)：3218-3219,3222,3.DOI:10.3969/j.issn.1009-5519.2014.21.007
红花注射液对脑缺血再灌注损伤大鼠大脑皮质Bcl-2及Bax表达的影响
Influence of safflower injection on expression of Bcl-2 and Bax protein in the cortex of rats after ischemia-reperfusion injury
摘要
Abstract
Objective To explore the influence of safflower injection on expression of Bcl-2 and Bax protein in the cor-tex of rats after ischemia-reperfusion injury. Methods Thirty-six healthy male SD rats were selected and randomly divided into sham-operation group,model group,low-dose group of safflower injection[(0.8 mL/(kg·d)],middle-dose group[1.6 mL/(kg·d)], high-dose group [3.2 mL/(kg·d)] and nimodipine injection group[(2.0 mL/(kg·d)],6 cases in each group. The sham-operation group and model group were managed with equal volume of normal saline instead. All the groups administrated with gastric injec-tion at 09:00 1 week before operation for 7 d continuously,once daily. To establish the focal cerebral ischemia-reperfusion injury model by thread technique;the expressions of Bcl-2 and Bax protein in the brain cortex of rats were detected by immunohisto-chemical methods. Results The positive expression of both Bcl-2 and Bax protein were significantly increased in the model group,compared with the negative result in the control group,the difference had statistical significance(P<0.05). Compared with the model group,the positive expression of Bcl-s in low-dose group,middle-dose group and high-dose group of safflower injection and nimodipine injection group increased obviously,the increase of the high-dose group of safflower injection was the most obvi-ous,and the difference was statistically significant (P<0.05),but the difference among low-dose group,middle-dose group and high-dose group had no statistical significance (P>0.05). Conclusion Safflower injection has protective effect on brain tissue of rats after ischemia-reperfusion injury by up-regulating Bcl-2 and down-regulating Bax to reduce apoptosis of cerebral cortical neurons.关键词
脑缺血/再灌注损伤/红花/注射剂/大脑皮质/原癌基因蛋白质类/基因,bcl-2Key words
Brain ischemia/Reperfusion injury/Carthamus tinctorius/Injectio/Cerebral cortex/Proto-onco-gene proteins/Genes,bcl-2引用本文复制引用
杨泽伟,冯飞,陈文明,杨小艳,罗勇..红花注射液对脑缺血再灌注损伤大鼠大脑皮质Bcl-2及Bax表达的影响[J].现代医药卫生,2014,(21):3218-3219,3222,3.基金项目
1.贵州省科学技术基金（黔科合J字[2009]2155号）；2.贵州省卫生厅科学技术基金项目（gzwkj2009-1-052）。 （）
