现代医药卫生Issue(22)：3374-3376,3.DOI:10.3969/j.issn.1009-5519.2014.22.007
离子色谱直接提取法测定天花粉中二氧化硫的含量
Content determination of Sulfur dioxide inTianhuafen by ion chromatography-direct extraction
余敏灵 1王小林 1陈文秋 1封家福2
作者信息
- 1. 乐山市食品药品检验检测中心，四川614000
- 2. 乐山职业技术学院，四川614000
- 折叠
摘要
Abstract
Objective To establish a quantitative analysis method to determine the content of the Sulfur dioxide (SO2) in Tianhuafen by ion chromatography-direct extraction. Methods The SO2 was extracted by potassium hydroxide solution(25 mmol/L) directly with AS11-HC chromatographic column(4 mm×250 mm,9.0μm);column temperature was 20℃;eluent was potassium hydroxide solution(20 mmol/L);the flow rate of eluent was 1.00 mL/min. Conductivity detection was adopted with the conductivity temperature of 20℃. The separating degree experiment,precision experiment and repetitive test were adopted to detect and calcu-late degree of separating,peak area of sulfite,content of SO2 in each gram of Tianhuafen and the relationship between peak area and sample volume. Results The chromatographic peak of sulfite had good separating degree with its adjacent chromatographic peak,and the peak area changed little;the mean content of sulfite was 0.197 7 mg in each gram of Tianhuafen with the RSD of 0.6%. When the injection quantity of sulfite sample was in the range of 1.16-29.1μg, which meant when the injection volume of sulfite was 100μL and the concentration range of solutions was within 11.6-291.2μg/mL,there was good linear relationship be-tween the injection quantity and peak area of sulfite. The average recovery rate was 99.2%,and the limit of quantification (LOQ) was 0.00 138μg/mL. Conclusion The method of ion chromatography-direct extraction is simple,accurate,rapid and appropriate for the quantification of SO2 in Tianhuafen.关键词
色谱法,离子交换/提取法/天花粉/二氧化硫/中草药Key words
Chromatography,ion exchange/Extraction/Radix trichosanthis/Sulfur dioxide/Drugs,chinese herbal引用本文复制引用
余敏灵,王小林,陈文秋,封家福..离子色谱直接提取法测定天花粉中二氧化硫的含量[J].现代医药卫生,2014,(22):3374-3376,3.
