现代医药卫生Issue(1)：3-5,8,4.DOI:10.3969/j.issn.1009-5519.2015.01.002
补体因子D及脂联素在子痫前期孕妇胎盘组织血清及尿液中的表达及相关性研究
The expression and correlative study of complement factor D and adiponectin in the placenta tissues,serum and urine in pregnancy with preeclampsia
摘要
Abstract
Objective To detect the expression and correlation of complement factor D (CFD) and adiponectin (ADPN) in the placenta tissues,serum and urine in pregnancy with preeclampsia. Methods It was selected 29 pregnant women with preeclampsia undergoing cesarean section (the preeclampsia group) and 30 normal full-term pregnant women with elective cae sarean section(the normal pregnancy group) admitted by the Department of Obstetrics of the First Affiliated Hospital of Chongqing Medical University from March to September in 2014. It was adopted Western blotting to detect the CFD and ADPN concentra-tions in placenta tissues and ELISA to detect the CFD and ADPN concentrations in serum and urine , calculating the two indicators′difference between the two groups and analyzing the correlation of the two. Results (1)The expression concentration of CFD protein of preeclamp sia patients was significantly increased in the placenta tissues compared to the normal pregnancy group(P<0.05 or 0.01). (2)The serum and urine concentrations of CFD in the normal pregnancy group were significantly lower than those in the preeclampsia group[(0.67±0.04)μg/mL,(43.27±16.59)ng/mL vs(1.83±0.59)μg/mL,(174.95±23.61)ng/mL, P<0.01];The serum and urine concentrations of ADPN in the normal pregnancy group were significantly higher than those in the preeclampsia group[(80.68±1.27),(9.29±1.33)ng/mL vs(13.16±2.01),(2.40±0.86)ng/mL,P<0.01]. (3) It showed a significantly negative correlation of CFD and ADPN concentrations in the placenta tissues ,serum and urine in pregnancy with preeclampsia (r=-0.82,-0.78,-0.63,P<0.05). Conclusion The abnormal changes of CFD and ADPN concentrations in the placental tissues , serum and urine in pregnant women may contribute to the pathology of preeclampsia ,and the two indicators may have synergistic effects. Taking advantage of combined detection of the CFD and ADPN concentrations in maternal serum or urine may be a promising method for prediction and diagnosis of preeclampsia.关键词
补体因子D/脂联素/子痫/脂肪因子类/胎盘/血清/尿/孕妇Key words
Complement factor D/Adiponectin/Eclampsia/Adipokines/Placenta/Serum/Urine/Pregnant women引用本文复制引用
潘亚萍,漆洪波,罗欣,刘西茹..补体因子D及脂联素在子痫前期孕妇胎盘组织血清及尿液中的表达及相关性研究[J].现代医药卫生,2015,(1):3-5,8,4.基金项目
国家自然科学基金（81300508、81300509、81471472）；广东省产科重大疾病重点实验室专项基金（粤科财字2011[102号]）。 （）
