现代医药卫生Issue(1)：14-15,18,3.DOI:10.3969/j.issn.1009-5519.2015.01.006
幽门螺杆菌重组热休克蛋白60对U937细胞分泌TNF-α和IL-17的影响
Effects of helicobacter pylori recombinant heat shock protein 60 on the secretion of TNF-αand IL-17 after stimulating U937 cells
摘要
Abstract
Objective To investigate the effects of helicobacter pylori recombinant heat shock protein 60 on the cell se-cretion of TNF-αand IL-17 of U937 cells. Methods Hsp60 and rhsp60 were expressed by gene mutation and recombination , which were co-cultured with 50,100,200μg/mL of rhsp60(rhsp60 group) and hsp60(hsp60 group) with U937 cells induced by phorbol myristate acetate(PMA). The final concentration of the groups was adjusted as 50 μg/mL,100μg/mL,200μg/mL. The supernatant was collected at 12th,18th,24th h and IL-17,TNF-αsecretion was measured by ELISA. The experiment was setted the phosphate buffer as the control group. Results The TNF-α,IL-17 secretion concentrations in the hsp60 and rhsp60 groups were significantly higher than those in the control group. The TNF-α,IL-17 secretion concentrations in rhsp60 group was higher than that in hsp60 group at the same time and the same concentration. The difference had statistical significance (P<0.05). Conclusion The rhsp60 and hsp60 could promote the secretion of IL-17,TNF-αand rhsp60 derived from gastric cancer had more effects than hsp60 after stimulating U937 cells. secretory cell factor TNF-αand IL-17 of U937 cells.关键词
螺杆菌,幽门/监控蛋白60/肿瘤坏死因子 α/白细胞介素17/重组,遗传/U937细胞/基因/突变Key words
Helicobacter pylori/Chaperonin 60/Tumor necrosis factor-alpha/Interleukin-17/Recombination, genetic/U937 cells/Genes/Mutation引用本文复制引用
张珍,魏义花,杜镇镇,周秀芝..幽门螺杆菌重组热休克蛋白60对U937细胞分泌TNF-α和IL-17的影响[J].现代医药卫生,2015,(1):14-15,18,3.基金项目
山东省医药卫生科技发展计划项目（2011HZ004）；山东省高等学校科技计划项目（J11LF73）。 （）
