现代医药卫生Issue(3)：323-325,328,4.DOI:10.3969/j.issn.1009﹣5519.2015.03.002
阿司匹林对结肠癌细胞肿瘤干性标记物CD44蛋白表达的影响
Treatment with aspirin inhibiting the expression of tumor stem marker CD44 in human colorectal cancer cells
摘要
Abstract
Objective To investigate the effects of aspirin affected by changes in the concentration of different drugs on CD44,which was supposed to be the marker of tumor stem cells in colon cancer cell line SW﹣480 and HT﹣29 so as to explore how to prevent the occurrence of colorectal cancer with aspirin. Methods The expression of CD44 in the colon cancer cell line SW﹣480 and HT﹣29 cells was observed by laser scanning confocal microscopy and immunofluorescence double staining. The expres﹣sion of CD44 protein in human colon cancer SW480(negative expression of COX﹣2) and HT﹣29(high expression of COX﹣2) cells with aspirin at different concentrations(0,2.5,5.0,10.0 mmol/L,namely the control group,the low﹣concentration group,the middle﹣concentration group and the high﹣concentration group) were detected by Western blotting. Results CD44+cells existed the SW﹣480 and HT﹣29 cells.There were statistical significance fluorescence value expressed by two kinds of cells. The difference had sta﹣tistical significance(P<0.05). Asprin could inhibit the expression of colon cancer cell line SW﹣480 and HT﹣29 by Western blotting, which showed an obvious dose﹣effect relationship (P<0.05). There were no statistical significance in difference between the two kinds of cells(P>0.05). Conclusion CD44+cells exist in the SW﹣480 and HT﹣29 cells. Aspirin may inhibit the expression of CD44 protein,the tumor stem maker in the colon cancer cell line SW﹣480 and HT﹣29,which was supposed to be acted by COX﹣2 and non COX﹣2 pathways. Asprin is probably one of factors to prevent colorectal cancer.关键词
阿司匹林/结肠肿瘤/肿瘤干细胞/CD44蛋白Key words
Aspirin/Colonic neoplasms/Neoplastic stem cells/CD44 protein引用本文复制引用
鲁生念,廖永美,赵逵..阿司匹林对结肠癌细胞肿瘤干性标记物CD44蛋白表达的影响[J].现代医药卫生,2015,(3):323-325,328,4.基金项目
贵州省高层次人才科研条件特助经费资助项目（TZJF﹣2011﹣32）。 （）
