现代医药卫生Issue(4)：481-484,4.DOI:10.3969/j.issn.1009-5519.2015.04.001
黄芪多糖联合顺铂对人肺癌A549细胞增殖抑制作用及其机制研究
Study on proliferation inhibition and its mechanism of astragalus polysaccharide combined with cisplatin on human lung cancer A549 cells
李蓉 1章运生 2谭小武1
作者信息
- 1. 南华大学附属第二医院呼吸内科，湖南衡阳421001
- 2. 南华大学附属第二医院临床研究所，湖南衡阳421001
- 折叠
摘要
Abstract
Objective To observe the inhibitory effects of astragalus polysaccharides and cisplatin on the cell prolifera-tion,cycle,apoptosis induction of human lung cancer A549 cells,and initially discuss its action mechanism. Methods The ef-fects on cell proliferation of astragalus polysaccharides ,cisplatin and their combinations was detected by MTT assay. It analyzed the impact of cell cycle and apoptosis rate by flow cytometry. The A549 human lung cancer cells Bax ,Bcl-2 and Caspase-3 gene expression levels by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Results Astragalus polysac-charide,cisplatin and two-drug combination on inhibition of proliferation of human lung cancer A549 cells had a time-concentra-tion-dependent proliferation. Compared with the negative control group ,the cell cycle blockage in G1 phase after astragalus polysaccharide action while the cell cycle blockage in S phase after cisplatin action and the application of two-drug combination appeared cell cycle blockage by flow cytometry both in G1 and S phases(P<0.01). The expression of Bax,Caspase-3 mRNA and pro-tein were upgraded in the expression of the two-drug combination group and lowered in Bcl-2 mRNA protein compared to the nega-tive control and monotherapy group. The difference had statistical significance (P<0.05). Conclusion The astragalus polysaccharide combined with cisplatin may strengthen human lung cancer A549 cells to enhance the pro-apoptosis,whose mechanism is associ-ated with upgrading the expression of Bax ,Caspase-3 and lowering the expression of Bcl-2mRNA.关键词
黄芪多糖/顺铂/半胱氨酸天冬氨酸蛋白酶3/人肺癌A549细胞/Bcl相关X蛋白/B细胞淋巴瘤-白血病2Key words
Astragalan/Cisplatin/Caspase 3/human lung cancer A549 cells/Bcl associated X protein/B cell lymphoma/leukemia-2引用本文复制引用
李蓉,章运生,谭小武..黄芪多糖联合顺铂对人肺癌A549细胞增殖抑制作用及其机制研究[J].现代医药卫生,2015,(4):481-484,4.
