摘要
Abstract
Objective To study the serological phenotype of D variants in volunteer blood donors initially-diagnosed Rh negative in Nanchong region of Sichuan province. Methods A total of 433 blood samples from 154 392 unrelated volunteer blood donors with Han nationality in Nanchong district of Sichuan province initially diagnosed as Rh negative were identified by indirect antiglobulin test for D variants ,and the negative results were subsequently detected by absorption-elution testing for DEL type from indirect antiglobulin testing. In addition ,all of the blood samples were screened and identified for irregular antibodies. Results Among 433 samples,402 cases(92.8%,402/433) were confirmed Rh negative and 23 cases(5.3%,23/433) were de-tected D variants,including DvccEe for 8 cases(34.8%,8/23),DvCcee for 6 cases(26.1%,6/23) and DvCCee for 3 cases(13.0%, 3/23);A total of 3 DEL phenotypes for 105 cases were found,including DelCcee for 87 cases(82.9%,87/105),DelCCee for 16 cases (15.2%,16/105),and DelCcEe for 2 cases(1.9%,2/105),all of which were associated with C antigen;Furthermore,irregular antibodies failed to be detected in plasma samples of donors with D variants and DEL type. Conclusion In the survey,serologi-cal phenotypes of D variant in volunteer blood donors initially-diagnosed Rh negative in Nanchong district of Sichuan province are learned,which has an important significance to guide the clinical blood transfusion and prevent hemolytic diseases of the newborns.关键词
供血者/血清学/四川/D变异体/DEL型/不规则抗体Key words
Blood donors/Serology/Sichuan/D variants/DEL type/Irregular antibody
