现代医药卫生Issue(6)：801-803,3.DOI:10.3969/j.issn.1009-5519.2015.06.001
肿瘤干细胞标志物在鼻咽癌细胞株中的表达
The expression of cancer stem cell markers in human nasopharyngeal carcinoma cell lines
刘观成 1何晓松 2宣广旭 2王文华 2陈文文1
作者信息
- 1. 桂林医学院，广西桂林541004
- 2. 桂林医学院附属医院耳鼻喉头颈外科，广西桂林541001
- 折叠
摘要
Abstract
Objective To explore the expression of cancer stem cells(CSC) markers named ABCG2,CD44,CD34 in hu man nasopharyngeal carcinoma(NPC) CNE-2,5-8F and 6-10B cell lines to provide the basis of CSC markers research of NPC. Methods The NPC cell lines of CNE2,5-8F and 6-10B were cultivated regularly,of which,ABCG2,CD44,CD34 cell ratio was detected with flow cytometry. Results ABCG2 positive cells accounted about 0.1%,18.6%and 19.9%in the CNE-2,5-8F,6-10B cell lines. CD44 positive cells accounted 99.5%,93.2%and 99.1%in the CNE-2,5-8F and 6-10B cell lines. CD34 positive cells accounted 0,3.0%and 0.1%in the CNE2,5-8F,and 6-10B cell lines. ABCG2+CD44+cell interation accounted about 11.6%and ABCG2+CD44+cell interation accounted about 0 in 5-8F cell lines. Conclusion The expression proportion of CD34+in the 5-8F and 6-10B cell lines confirms to CSC markers of the CSC theory. CD34 may be deemed as a candidate marker of nasopharyn-geal neoplas.关键词
肿瘤干细胞/生物学标记/鼻咽肿瘤/病理学/细胞系/表达/交互作用Key words
Neoplastic stem cells/Biological markers/Nasopharyngeal neoplasms/pathology/Cell line/Ex-press/Interaction引用本文复制引用
刘观成,何晓松,宣广旭,王文华,陈文文..肿瘤干细胞标志物在鼻咽癌细胞株中的表达[J].现代医药卫生,2015,(6):801-803,3.
