现代医药卫生Issue(6)：820-822,3.DOI:10.3969/j.issn.1009-5519.2015.06.008
氯化镍对真核小鼠胚胎成纤维细胞超氧化物歧化酶2的影响及其机制
Effect with NiCl2 on SOD2 in MEF eukaryotic cells and its mechanisms
摘要
Abstract
Objective In MEF eukaryotic cells,it was stimulated with NiCl2 with different concentrations and times. It is investigated the change of SOD2 and its potential mechanisms. Methods Cultivating MEF cells line and preparing Nicl2 solu-tions, it was observed the change of SOD2 stimulated by NiCl2 of different concentrations and times by Western blotting and then judged the potential mechanism. Results After stimulating MEF for continuous 12 hours with different-concentration NiCl2,the expression of SOD2 was orientated to declining and p-Stat3 and p-Akt rising respectively in general. After stimulating MEF for dif-ferent time period with the same-concentration NiCl2,the expressions of SOD2 and p-Stat3 were both orientated to decline and ris-ing respectively generally. The overall change of SOD2 and p-Stat3 were in consistent. Conclusion Stimulated by NiCl2,SOD2 showed a trend of decline,p-Stat3, probably as the transcription factor,adjusted SOD2 on the transcriptional level.关键词
镍/超氧化物歧化酶/成纤维细胞/基因表达/氯化镍Key words
Nickel/Superoxide dismutase/Fibroblasts/Gene expression/Nickel chloride引用本文复制引用
王盼盼,常文辉,骆文静,陈景元,杜可军..氯化镍对真核小鼠胚胎成纤维细胞超氧化物歧化酶2的影响及其机制[J].现代医药卫生,2015,(6):820-822,3.基金项目
国家自然科学基金资助项目（30972429）；教育部长江学者创新团队计划项目（PCSIRT1112）；中国博士后课题资助项目（20060391015）。 （）
