现代医药卫生Issue(7)：982-985,4.DOI:10.3969/j.issn.1009-5519.2015.07.009
高效液相色谱法测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度评定
Uncertainty evaluation of determination of vitamin B6 content in oyster soybean peptide carnitine oral liquid by high per- formance liquid chromatography
庞学斌 1张伟 1邱颖姮 1邬晓鸥1
作者信息
- 1. 深圳药品质量标准研究重点实验室/深圳市药品检验所，广东518057
- 折叠
摘要
Abstract
Objective Uncertainty evaluation of determination of vitamin B6 content in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography(HPLC assay). Methods HPLC was adopted for the determination of vitamin B6 content. Mathematical model was established for the evaluation of uncertainty to quantize influential factors of uncertainty and evaluate the uncertainty factors further. Results The content of vitamin B6 in sample oral liquid was 0.498 mg/100mL,in which, the relative uncertaintyof vitamin B6 content was 0.024 9,the standard uncertainty was 0.0124 mg/100 mL,the expanded uncertainty was 0.024 8 mg/100 mL(k=2). Conclusion The calculation method is practical and reliable,being access to the uncertainty eval—uation of content of vitamin B6 in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography. Upon the evaluation,it is found that the quantitative repetitive of the HPLC is the major impact.关键词
色谱法,高压液相/维生素B6/牡蛎/数学计算Key words
Chromatography,high pressure liquid/Vitamin B6/Oysters/Mathematical computing引用本文复制引用
庞学斌,张伟,邱颖姮,邬晓鸥..高效液相色谱法测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度评定[J].现代医药卫生,2015,(7):982-985,4.
