现代医药卫生Issue(10)：1445-1447,3.DOI:10.3969/j.issn.1009-5519.2015.10.002
OsLEA5蛋白对乳酸脱氢酶体外保护机制的研究
A study of protection mechanism of OsLEA5 proteins on lactic dehydrogenase(LDH) in vitro
摘要
Abstract
Objective To determine the protective effect of late embryogenesis abundant (LEA) protein on enzyme ac-tivity under different stresses in vitro and discuss its potential mechanism. Methods The protective capability of OsLEA5 pro-teins to prevent lactic dehydrogenase(LDH) from high temperature(43℃),multigelation and dehydration was tested. Phosphate buffer,BSA and pure water were respectively used as positive and negative controls. Results The percentage of OsLEA5 proteins′relative activity was 2.2,3.7,5.4 times higher than pure water′s after 10,20,30 min of heat treatment. Pure water′s LDH activity dropped to 7%or even lower after 5 multigelation cycles while OsLEA5 proteins with a mass ratio of 2:1 and 5:1 were 28%and 69%,and OsLEA5 protein with a mass ratio of 10∶1 remained uninfluenced. The LDH activity of different mass ratios clearly dropped after drying treatment,in which pure water and phosphate buffer′s LDH was almost completely inactivated,and OsLEA5 proteins with a mass ratio of 5∶1 and 10∶1 was 24%and 36%. Conclusion OsLEA5 proteins effectively protect substrate enzyme′s activity in vitro and prevent inactivation under various stress conditions.关键词
L-乳酸脱氢酶/植物蛋白质类/高温,诱发/反复冻融/脱水Key words
Lactate dehydrogenases/Plant proteins/Hyperthermia,induced/Multigelation/Dehydration引用本文复制引用
何帅,胡廷章..OsLEA5蛋白对乳酸脱氢酶体外保护机制的研究[J].现代医药卫生,2015,(10):1445-1447,3.基金项目
教育部“春晖计划”（Z2007-1-63006）；中国博士后科学基金（No.20070420717）。 （）
