现代医药卫生Issue(12)：1764-1766,1769,4.DOI:10.3969/j.issn.1009-5519.2015.12.002
G250mAb修饰的肿瘤细胞靶向基因载体功能研究
Study on vector function of G250 mAb modified tumor cell target gene
摘要
Abstract
Objective To research and analyze the superiority of G250mAb coupling with polyethyleneimine (PEI) as a tumor-targeted gene vector to lay the foundation for targeted gene therapy of tumor cells. Methods G250mAb was coupled with PEI by the disulfide bond for obtaining the modified gene vector G250mAb-PEI. The transfected cells in vitro method was applied to study its transgenetic superiority. Results The transfection efficiency of G250mAb-PEI was significantly higher than that of pure PEI or traditional lipofectamine trasfection;its toxicity was lower than that of non-modified PEI;the gene transfection of cer-vical cancer Hela cells had significant targeting and its transfection efficiency was twice that of liver cancer cells HepG2 (G250 negative),but the transfection efficiency of normal vascular smooth muscle cells (SMC) was lower than that of Hela by nearly 20-fold. Conclusion G250mAb-PEI is a highly efficient gene vector with low cytotoxicity and targeting effect.关键词
Hela细胞/聚乙烯亚胺/遗传载体/肿瘤/病理学/基因/转染/G250mAbKey words
Hela cell/Polyethyleneimine/Genetic vectors/Neoplasms/pathology/Genes/Transfection/G250mAb引用本文复制引用
李廷,徐涛,张延辉,刘春雷..G250mAb修饰的肿瘤细胞靶向基因载体功能研究[J].现代医药卫生,2015,(12):1764-1766,1769,4.基金项目
青岛市卫生科技发展计划项目（WSZD080）。 （）
