现代医药卫生Issue(15)：2259-2261,3.DOI:10.3969/j.issn.1009-5519.2015.15.004
人成骨肉瘤MG63紫杉醇耐药细胞系建立及生物学特性研究
Study on establishment of paclitaxel resistant osteosarcoma cell line MG63/Taxol and its biological characteristics
摘要
Abstract
Objective To establish the paclitaxel(Taxol0 induced osteosarcoma MG63 drug resistance cell subline(MG63/Taxol )and to observe its biological characteristics. Methods Paclitaxel resistant human osteosarcoma cell subline (MG63/Tax-ol) was established by adopting large dose impact combined with small dose maintenance to induce MG63 cell line with Taxol as the inducer. The sensitivity of chemotherapy drugs for the MG63 and MG63/Taxol cell sublines were measured by the colony for-mation assay. The cell morphology change was observed through light microscopy. The proliferation ability of cell lines was mea-sured by growth curve. The cell stages were analyzed by flow cytometry. Results (1)Through 6-month induction,paclitaxel re-sistant osteosarcoma cell subline(MG63/Taxol)was established. (2)The resistant index of MG63/Taxol to Taxol was 10.55. MG63/Taxol also produced the various degrees of cross resistance to cisplatin , methotrexate and 5-Fu. (3)The cell morphology was ob-served through light microscopy. The MG63/Taxol cells were regular arranging, spindle shape and size consistent,MG63/Taxol cell subline was irregular arranging,size inconsistent and morphology showed triangle shape,multiangular shape and multi-nucle-ation.(4)The cell growth cure showed that MG63/Taxol cells grew slowly than the parent cells. (5)The cell cyclical analysis dis-played that the distribution of the G0/G1 stage cells was increased ,while the S stage cells were decreased. Conclusion (1)A pa-clitaxel resistant human osteosarcoma cell subline (MG63/Taxol) established by adopting the large dose impact combined with small dose maintenance(MG63)generates the cross resistance to cisplatin,methotrexate and 5-Fu in different degrees.(2)MG63/Taxol cell subline is significantly different from the parent cell MG63 in the aspects of cell morphology,cell growth curve and cell cycle distribution.关键词
骨肉瘤/细胞系/细胞凋亡/细胞增殖/药物耐受性/紫杉醇/耐药细胞Key words
Osteosarcoma/Cell line/Apoptosis/Cell proliferation/Drug tolerance/Paclitaxel/Resistant cells引用本文复制引用
吴文欣,李维..人成骨肉瘤MG63紫杉醇耐药细胞系建立及生物学特性研究[J].现代医药卫生,2015,(15):2259-2261,3.基金项目
湖南省自然科学基金资助项目（11JJ3105）；湖南省科技计划项目（2013FJ3039）。 （）
