协和医学杂志Issue(3)：264-269,6.DOI:10.3969/j.issn.1674-9081.2014.03.004
老年2型糖尿病患者血清胆固醇酯脂肪酸检测
Detection of Serum Cholesterol Ester Fatty Acids in the Elderly with Type 2 Diabetes Mellitus
摘要
Abstract
Objective To evaluate the alteration of cholesterol ester fatty acids and related serum biochemical parameters in elderly patients with type 2 diabetes mellitus ( T2DM).Methods Totally 73 T2DM patients (T2DM group) and 72 healthy subjects (control group) (aged ≥60 years) who received health check-ups in Beijing Hospital from August to December in 2012 were recruited and blood samples were collected .Eleven main cholesterol ester fatty acids in serum were determined with liquid chromatography tandem mass spectrometry , and endogenous conversions by desaturases and elongases were estimated from product -to-precursor ratios .Fasting plas-ma glucose (FPG), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein (Apo) AI (ApoAI), ApoB, and high sensitivity C-reactive protein (hsCRP) were analyzed with an automatic biochemistry analyzer and the results were statisti -cally analyzed .Results CE14∶0, CE16∶1 and CE22∶6 in T2DM group were significantly lower than those in the control group ( P <0.05 ) , while no significant difference existed between other fatty acid compositions .Δ9-desaturase activity was lower in T2DM group compared with that in control group (P=0.001).After adjus-ting for age, gender, body mass index (BMI), TC, TG, and FPG, Logistic analysis showed the quartiles of CE22∶6 andΔ9-desaturase activity were associated with risk of type 2 DM, and the odds ratios were 0.27 (95%CI:0.08-0.86, Wald:4.93, P<0.05) and 0.06 (95%CI:0.01-0.22, Wald: 17.39, P<0.01), respec-tively, for comparison of the top to bottom quartile .Both CE14∶0 and CE20∶3 negatively correlated with FPG (r=-0.18, -0.17, P<0.05);CE18∶0 positively correlated with BMI and TG (r=0.25, 0.24, P<0.01) and negatively correlated with HDL-C and ApoAI (r=-0.39, -0.35, P<0.01);CE20∶3 positively correlated with BMI, with a correlation coefficient of 0.42 (P<0.01).Conclusions Several cholesterol ester fatty acids de-crease in elderly T2DM patients.CE22∶6 andΔ9-desaturase may protect against T 2DM.An appropriately balanced intake of fatty acids is helpful for the prevention of T 2DM in the elderly population .关键词
脂肪酸/2型糖尿病/脂肪酸脱氢酶Key words
fatty acids/type 2 diabetes mellitus/desaturase分类
医药卫生引用本文复制引用
禹松林,陈文祥,董军,周伟燕,杨睿悦,李红霞,张顺利,张天娇,赵海建,张传宝,王抒..老年2型糖尿病患者血清胆固醇酯脂肪酸检测[J].协和医学杂志,2014,(3):264-269,6.基金项目
国家自然科学基金 （）
