新疆农业科学Issue(2)：334-338,5.DOI:10.6048/j.issn.1001-4330.2015.02.024
新疆部分地区牛病毒性腹泻病毒的分离﹑鉴定及分子流行病学调查
Bovine Viral Diarrhea Virus Isolation Identification and Molecular Epidemiology Investigation in some Areas in Xinjiang
摘要
Abstract
Objective]In order to understand the epidemic of bovine viral diarrhea (BVD) in Xinjiang region.[Method]5′—untranslated region of BVD virus from different 16 areas of Xinjiang were amplified by RT —PCR,building genetic evolutionary tree and analyzing their differences.[Result] According to the re_sults,there were 21 positive out of 566 samples,the average positive rate was 3.71%.17 BVDV —I type and 4 BVDV —II type strains were found after blasting their homologies.Those positive samples were cultured with cells,7 strains were Cytopathic biotype(CP),14 strains were Non —cytopathic biotype(NCP),the virus titers ranged from 1.04 x 103 to 6.02 x 108 TCID50 ∕0.1ml,the 21 positive serum neutralization titers were from1:21 to 1:25 .[Conclusion]The results showed that there were varying degrees of incidence and prevalence of the BVD in Xinjiang.All of those were providing technical basis for the prevention and control of BVD.关键词
BVDV/序列分析/分离鉴定/分子流行病学调查Key words
BVDV/gene sequence analysis/isolation and identification/molecular epidemiological inves_tigation分类
农业科技引用本文复制引用
王龙,梁霄勇,季新成,史茜,冉多良..新疆部分地区牛病毒性腹泻病毒的分离﹑鉴定及分子流行病学调查[J].新疆农业科学,2015,(2):334-338,5.基金项目
新疆维吾尔自治区高新技术项目(201010101,201141147)；国家质量监督检验检疫总局科研项目(2011IK017) （201010101,201141147）
