新疆农业科学Issue(2)：349-353,5.DOI:10.6048/j.issn.1001-4330.2015.02.027
增强型绿色荧光蛋白eGFP基因与犬瘟热N蛋白基因重组质粒的构建
Construction of Recombinant Plasmid with Enhanced Green Fluorescent Protein Gene eGFp and N Protein Gene of Canine Distemper Virus
摘要
Abstract
Objective]The recombinant plasmid of enhanced green fluorescent protein gene eGFP and N protein gene in canine distemper virus was constructed for the infectious clone of recombinant canine distemper virus in this paper.[Method]The recombinant PCR primers for eGFP gene and CDV N gene were designed and amplified.The amplified products were fused again by recombinant PCR.[Result]The fragments of eGFP gene and CDV N gene were 720 bp and 1 572 bp,and homology were 100% and 99.81%,respectively,com_pared with the GenBank eGFP (ID: X83959)and CDV N(ID:AY466011).The size of fusion gene eGFP —CDV N was 2 292 bp,consistently with the expected one.[Conclusion]The recombinant plasmid of T —eGFP—CDVN was successfully constructed.It can be used for the expression of fusion gene eGFP —CDV N.关键词
重组PCR/eGFP基因/犬瘟热N蛋白/重组基因Key words
recombinant PCR/enhanced green fluorescent protein gene/canine distemper virus N/recom_binant gene分类
农业科技引用本文复制引用
夏婷婷,苏晓慧,翟少华,简子健..增强型绿色荧光蛋白eGFP基因与犬瘟热N蛋白基因重组质粒的构建[J].新疆农业科学,2015,(2):349-353,5.基金项目
国家自然科学基金项目(31360623)；新疆维吾尔自治区普通高校重点学科(基础兽医学)科研启动基金 （31360623）
