新疆农业科学Issue(2)：354-359,6.DOI:10.6048/j.issn.1001-4330.2015.02.028
移植脐带间充质干细胞对荷斯坦公犊生长性能的影响
Transplantation of Umbilical Cord Mesenchymal Stem Cells on the Growth Performance of Holstein male Calves
摘要
Abstract
[Objective] To investigate the effect of transplantation of umbilical cord mesenchymal stem cells on the growth performance of Holstein bull calves.[Method]From July 2013 to December,28 Holstein calves with similar ages and weights were selected and randomly divided into control and experimental groups. The successfully —cultured umbilical cord mesenchymal stem cells were diluted to 8ml according to body weight of male calf 3 x 105 and injected into the jugular vein in vivo.The subjects in the control group were injected with saline.Body fasting body weight,body height and length,heart girth and cannon circumference tube angle width of the subjects in both groups were measured.The subjects in both groups were measured and blood was drawn and centrifuged at 3,500 rpm and kept for 15 min.These kinds of experiments were repeated 6 times.[Result]The test successfully cultured umbilical cord mesenchymal stem cells; When the calves were 4,5 and 6 months of age,the average weight of the experimental group was higher (P ﹤0.05) than that of the control group; At 4 months of age,the daily average gain of the experimental group was higher than that of the control group (P ﹤0.05); At 6 months of age,the daily average gain of the experimental group was signifi_cantly higher than that of the control group g (P ﹤0.01).Various indexes like body size of the test group at the peak of the growth and development of male calves were higher,and the difference was significant (P ﹤0.05).P content in the test serum at 3,4 and 6 months of age was significantly higher (P ﹤0.05).Test ser_um Ca content of 5,6 months old at the time was significantly higher (P ﹤0.05).[Conclusion]Injecting um_bilical cord mesenchymal stem cells could significantly increase the growth rate of Holstein bull calves and ef_fectively enhance their growth performance.关键词
脐带间充质干细胞/荷斯坦公犊/生长性能Key words
umbilical cord mesenchymal stem cells/Holstein male calves/growth performance分类
农业科技引用本文复制引用
赵艳坤,邵伟,王立文,彭国亮,余雄..移植脐带间充质干细胞对荷斯坦公犊生长性能的影响[J].新疆农业科学,2015,(2):354-359,6.基金项目
“十二五”国家科技支撑项目“牧区及农牧交错带奶牛健康养殖技术集成及产业化示范”(2012BAD12B09)；“新疆肉牛安全高效饲养关键技术研究与示范”(2011BAD47B02)；“不同用途马高效﹑安全养殖共性技术研发”(2012BAD45B01)；自治区重大专项“新疆农区奶牛健康养殖及疫病防控技术推广与示范”(20101230694)；2013年度新疆研究生科研创新项目(xjau -2013-yjsky -XJGRI2013113)；新疆肉乳用草食动物营养实验室开放课题资助 （2012BAD12B09）
