新疆师范大学学报（自然科学版）Issue(2)：22-26,5.
独行菜eEF-1 a基因片段分离克隆及RT-P CR分析
Cloning and RT-PCR Analysis of eEF-1a Gene Fragment from Lepidium (Lepidium apetalum Willd.)
摘要
Abstract
Degenerate primers were designed based on the conserved sequences of the eEF-1a genes from oth-er plants. And the eEF-1a gene fragment was obtained by RT- PCR from Lepidium (Lepidium apetalum Willd.). The eEF-1a gene fragment contained 570 bp nucleotide acids. Blasting analysis showed that it has 95% nucleotide sequence homology with one in arabidopsis thaliana. And RT-PCR analysis showed that the expression of eEF-1a gene was steady in Lepidium, and it can be used as internal standard when study on other genes’ expression and regulation in Lepidium.关键词
eEF-1a( eEF1A)基因/克隆/RT-PCR分析Key words
Lepidium (Lepidium apetalum Willd.)/eEF-1a(eEF1A) gene/Clone/RT-PCR analysis分类
生物科学引用本文复制引用
葛凤伟,田永芝,曾卫军,赵惠新..独行菜eEF-1 a基因片段分离克隆及RT-P CR分析[J].新疆师范大学学报（自然科学版）,2014,(2):22-26,5.基金项目
新疆维吾尔自治区自然科学基金项目（2012211A054）。 （）
