西南国防医药Issue(7)：697-700,4.DOI:10.3969/j.issn.1004-0188.2014.07.001
侵袭性白念珠菌小鼠感染模型的建立与评估
Establishment and evaluation of invasive Candida albicans infection model in mice
摘要
Abstract
Objective To establish an invasive Candida albicans infection model in mice. Methods The virulence of Candida albicans in vitro was evaluated by means of mycelial growth experiment,while that in vivo was evaluated through the mouse infection model;an aliquot of culture containing Candida albicans with 5 × 105 CFU was injected into each mouse through the tail vein and the survival time of the infected mice was observed. 100% death of the mice within 7 days of infection was used as the successful establishment of the infection model;fluconazole was applied to the infected mice and the effect on the infection of Candida albicans was evaluated as well. Results Candida albicans had the same virulence in vitro and in vivo;strains were well inducted in the 4 hyphal induction media;the survival rate of the infected mice was very low;fluconazole was more effective on Candida albicans sensitive to it than on those resistant to it( P ﹤ 0. 05). Conclusions Invasive Candida albicans infection model in mice is applicable for drug screening of anti-Candida albicans activity in vivo.关键词
白念珠菌/毒力/感染/小鼠/氟康唑/体内实验Key words
Candida albicans/virulence/infection/mice/fluconazole/in vivo分类
医药卫生引用本文复制引用
杜发娅,阎澜,姜远英,徐贵丽..侵袭性白念珠菌小鼠感染模型的建立与评估[J].西南国防医药,2014,(7):697-700,4.基金项目
国家自然科学基金资助项目 （）
