西南国防医药Issue(8)：813-815,3.DOI:10.3969/j.issn.1004-0188.2014.08.001
pEGFP-N1-UCH-L1重组质粒的构建
Construction of recombinant plasmid pEGFP-N1-UCH-L1
摘要
Abstract
Objective To construct and identify the eukaryotic expression vector for UCH-L1 gene. Methods Total RNA was extracted from newborn Wistar rats and reversely transcribed to cDNA,with which UCH-L1 gene was amplified by RT-PCR and cloned into the eukaryotic expression vector pEGFP-N1;the constructed recombinant plasmid was identified by restriction analysis and sequencing. Results The results of restriction analysis and sequencing proved that UCH-L1 gene is successfully inserted into plasmid pEGFP-N1. Conclusions The eukaryotic expression vector for UCH-L1 is successfully constructed,and can be used to transfect neurons in further study.关键词
泛素羧基末端水解酶 L1/pEGFP-N1载体/重组质粒Key words
UCH-L1/pEGFP-N1 vector/recombinant plasmid分类
医药卫生引用本文复制引用
卢豪,庞伟,徐婷,杨红澎,奚用勇,黄承钰,蒋与刚..pEGFP-N1-UCH-L1重组质粒的构建[J].西南国防医药,2014,(8):813-815,3.基金项目
国家自然科学基金（30872098，30901185）；天津市自然科学基金（05YFJMJC05500）；全军医学科技青年培育项目 （）
