西南国防医药Issue(10)：1055-1057,3.DOI:10.3969/j.issn.1004-0188.2014.10.005
HPLC 法测定芬戈莫德胶囊的含量
Determination of contents of fingolimod in fingolimod capsules by HPLC
摘要
Abstract
Objective To establish a method for determining the contents of fingolimod in fingolimod capsules by High Performance Liquid Chromatography( HPLC). Methods HPLC was used to determine the contents of fingolimod in fingolimod capsules. The selected column was Agilent TC-C18,and the mobile phase was sodium perchlorate solution(0. 1 mol/ L aqueous solution of sodium perchlorate,and the pH value of perchloric acid had been adjusted to 2. 8):methanol :acetonitrile(93:7:100);the detection wavelength was 215 nm with a flow rate of 1. 5 ml/ min;the sample size is 10 μl. Results The linear range of concentration of fingolimod was 0. 0499 ~ 0. 1499 mg/ ml(r = 0. 9998);the average recovery rate of samples was 100. 01%(RSD = 0. 46% ,n = 3). Conclusion This method is simple with high reproducibility and high accuracy,and can be used to determine the contents of fingolimod in fingolimod capsules for quality control.关键词
芬戈莫德/含量/测定/高效液相色谱法Key words
fingolimod/contents/determination/HPLC分类
医药卫生引用本文复制引用
王伟,路海滨..HPLC 法测定芬戈莫德胶囊的含量[J].西南国防医药,2014,(10):1055-1057,3.基金项目
吉林省医药产业发展专项资金资助课题 （）
