西南林业大学学报Issue(4)：20-25,6.DOI:10.3969/j.issn.2095-1914.2014.04.004
白桦开花调控相关基因FLC、LFY和SOC1的克隆及表达分析
Cloning and Expression Analysis of Flowering Regulation Related Genes FLC、LFY and SOC1 of Betula platyphylla
摘要
Abstract
LFY,FLC and SOC1 genes are key node genes in flowering regulation network.To characterize their function in birch,complete cDNA sequences of LFY and FLC were obtained from transcriptome datas of Betula platyphylla,named BpLFY and BpLFY,respectively.BpLFY was 1 215 bp in length,encoding a protein of 405 a-mino acid residues with the molecular mass of 45 .3 kD.The length of BpLFY gene cDNA was 627 bp,encoding 209 amino acid residues with the molecular mass of 1 1.4 kD,the two genes belonged to FLO-LFY and MADS su-perfamily,respectively.Analysis of promoters of these genes showed that promoters of all these genes contained two cis-acting elements of pollen specific expression.In different tissues (shoot tip,leaf,male inflorescence and axilla-ry),gene expression of BpLFY,BpFLC and BpSOC1 genes were investigated by using real-time quantitative RT-PCR.The results showed that BpSOC1 and BpFLC were expressed in various tissues of birch,however,BpLFY was expressed only in the male inflorescence and axillary,and BpFLC can inhibit BpSOC1 expression in the leaves.关键词
白桦/FLC/LFY/SOC1/启动子分析/基因表达Key words
Betula platyphylla/FLC/LFY/SOC1/analysis of promoter/gene expression分类
农业科技引用本文复制引用
王子佳,姜静,刘桂丰,刘菲菲,李慧玉..白桦开花调控相关基因FLC、LFY和SOC1的克隆及表达分析[J].西南林业大学学报,2014,(4):20-25,6.基金项目
“十二五”农村领域国家科技计划课题（2013AA102704）资助。 （）
