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变栖克雷伯菌在石斛体内的定殖动态及其对石斛黑斑病的防效试验

赵明富 李梦娇 张芬芬 孙媛 郭李川 宋爽 余代宏 文国松 徐绍忠

西南林业大学学报Issue(3):14-19,6.
西南林业大学学报Issue(3):14-19,6.DOI:10.3969/j.issn.2095-1914.2015.03.003

变栖克雷伯菌在石斛体内的定殖动态及其对石斛黑斑病的防效试验

Colonization of Klebsiella variicola in Dendrobium officinale and Its Control Effect on Dendrobium alternaria

赵明富 1李梦娇 1张芬芬 2孙媛 1郭李川 2宋爽 2余代宏 3文国松 2徐绍忠2

作者信息

  • 1. 云南农业大学植物保护学院,云南 昆明 650021
  • 2. 云南农业大学云南省中药材规范化种植技术指导中心,云南 昆明 650021
  • 3. 元江县农科所,云南 玉溪 653300
  • 折叠

摘要

Abstract

In this paper,made bacterial strain Klebsiella variicola SH -1 which was selected before as materi-al,explored colonization trends of Klebsiella variicola SH -1 on Dendrobium alternaria by rifampicin labeling,and then potting and greenhouse trials were conducted to discuss its control efficiency for Dendrobium alternaria.The results showed that SH -1 could colonize in Dendrobium,and the colonization trended to increase firstly and then decrease.After inoculation,colonization quantity reached the highest at 11d,15d and 20d,in roots,stems and leaves respectively,and then followed by a downward trend.Kept the relatively stable colonization level in Den-drobium,with 4.3 ×104 CFU /g,2.32 ×104 CFU /g and 1.84 ×104 CFU /g in roots,stems and leaves after 35d, respectively.The result of potted tests on prevention of the Dendrobium alternaria showed that SH -1 had obvious control effect on Dendrobium alternaria,the control effect was the same as conventional medicine Dimetachlone that the relative control effect reached 72%,which could reach 71.53%.

关键词

变栖克雷伯/定殖动态/石斛黑斑病/防治

Key words

Klebsiella variicola/colonization trends/Dendrobium alternaria/prevention

分类

农业科技

引用本文复制引用

赵明富,李梦娇,张芬芬,孙媛,郭李川,宋爽,余代宏,文国松,徐绍忠..变栖克雷伯菌在石斛体内的定殖动态及其对石斛黑斑病的防效试验[J].西南林业大学学报,2015,(3):14-19,6.

基金项目

国家“十二五”科技支撑计划项目(2011BAI13B01-02-03)资助;农业科技成果转化资金项目(2013GB2F300441)资助。 ()

西南林业大学学报

OA北大核心CSTPCD

2095-1914

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