西南林业大学学报Issue(3)：40-46,7.DOI:10.3969/j.issn.2095-1914.2015.03.008
橡胶树乳管表达多酚氧化酶基因 HbPPO1的克隆与分析
Molecular Cloning and Analysis of a Polyphenol Oxidase Gene (HbPPO1)from the Hevea brasiliensis Laticifer
摘要
Abstract
Based on the EST sequence,a 2 102 bp cDNA was isolated from the rubber tree (Hevea brasilien-sis)latex (representing the laticicfer cytoplasm)with specific primers via RACE and RT -PCR.The sequence designated as HbPPO1 contains a 1 803 bp long open reading frame (ORF),81 bp 5′UTR and 218 bp 3′UTR. Sequence analysis indicated that HbPPO1 putatively encodes 600 amino acids with a theoretical molecular weight (Mw)of 67.88 kDa and isolectric point (pI)of 8.56,which was predicted to be localized to the plastid.The pro-tein contains the conserved Tyrosinase,PPO1 _DWL and PPO1 _KFDV domains specific to polyphenol oxidases, and shares the similarity of 85.2%,82.0%,79.8% and 78.2% with its homologs in Manihot esculenta,Ricinus communis,Jatropha curcas and Populus euphratica.Expression analysis showed that,among the examined tissues such as laticifer,bark and leaf,HbPPO1 expressed relatively more in the laticifer and the expression level was sig-nificantly inhibited by ethylene.关键词
橡胶树/多酚氧化酶/乳管/胶乳/基因Key words
Hevea brasiliensis/polyphenol oxidase/laticifer/latex/gene分类
农业科技引用本文复制引用
邹智,谢贵水,莫业勇,王丹华,杨礼富..橡胶树乳管表达多酚氧化酶基因 HbPPO1的克隆与分析[J].西南林业大学学报,2015,(3):40-46,7.基金项目
国家自然科学资金（31371556）资助；海南省自然科学基金（312026）资助；现代农业产业技术体系建设专项资金（CARS －34－GW5）资助。 （）
