湖北科技学院学报（医学版）Issue(3)：188-190,196,4.
吡格列酮抑制体外高糖培养乳鼠心肌细胞炎症作用的研究
Pioglitazone Inhibits High Glucose-induced Inflammation in Neonatal Rat Cardiac Myocytes
摘要
Abstract
Objective To investigate the effect of pioglitazone on high glucose-induced inflammation in neonatal rat cardiac myocytes .Methods Ventricular myocytes were isolated from neonatal rats and cultured in DMEM medium.All the cells were randomly divided into 3 groups:control group,high glucose group,tpioglita-zone low-dose group and pioglitazone high-dose group .The levels of LDH ,IL-6 and TNF-αin cardiac myocytes were determined.At the same time,the expression of p38MAPK and NF-κB protein was examined by Western blot .Results High glucose induced obvious injury of cardiac myocytes ,increased the contents of LDH ,IL-6 and TNF-αand elevated the expression of p 38MAPK and NF-κB protein.Treatment with pioglitazone could signifi-cantly inhibite high glucose-induced inflammatory injury ,decrease the contents of LDH ,IL-6 and TNF-α,and reversed the elevation of p 38 MAPK and NF-κB protein expression induced by high glucose .Conclusion Piogli-tazone can inhibit the inflammatory reactionof cultured neonatal rat cardiac myocytes induced by high glucose .关键词
吡格列酮/原代心肌细胞/炎症/高糖Key words
Pioglitazone/Neonatal rat cardiac myocytes/Inflammation/Highglucose分类
医药卫生引用本文复制引用
胡梅,鲍翠玉,骆晓艳..吡格列酮抑制体外高糖培养乳鼠心肌细胞炎症作用的研究[J].湖北科技学院学报（医学版）,2015,(3):188-190,196,4.
