刑事技术Issue(3)：242-243,2.DOI:10.16467/j.1008-3650.2015.03.018
检材采集与保存方式对DNA提取效率的影响
Effect of Sample Collection and Preservation on DNA Yield
邓淑娇 1李萍 2黄桂清 1杨电3
作者信息
- 1. 广州市公安局白云区分局，广州 510420
- 2. 广州市公安局越秀区分局，广州 510080
- 3. 广州市公安局，广州 510030
- 折叠
摘要
Abstract
ABSTRACT:ObjectiveTo explore the effect of sample collection and preservation on DNA yield.MethodsThe fresh anti-freezing blood from 30 volunteers was respectively dropped onto a swab from the casing for collection and preservation of biological sample, and the ones of both the cuspidal and the common medical. Each of the blood specimens was separately preserved in the casing or paper bags for a week. Every piece of DNA from the blood specimens was extracted by magnetic beads method in combination of automation workstation and was quantiifed by ABI 7500 Real Time System.ResultsThe average DNA concentration was (2.54±0.63) ng/μL for the blood specimens collected and preserved with the casings, (2.06±0.44) ng/μL and (0.93±0.59) ng/μL for those blood specimens which were collected with cuspidal swabs and common medical ones and followed to preserve in paper bags. The DNA yield was obviously higher using the casings than that of either the cuspidal swabs or the common medical ones to collect and then preserve in paper bags.ConclusionsThis test suggests that it is better to choose the casings for collection and preservation of biological sample than the cuspidal and the common medical swab.关键词
法医物证学/生物检材采集/生物检材保存/DNA产量Key words
forensic biological evidence/biological sample collection/biological sample preservation/DNA yield分类
社会科学引用本文复制引用
邓淑娇,李萍,黄桂清,杨电..检材采集与保存方式对DNA提取效率的影响[J].刑事技术,2015,(3):242-243,2.
