信阳师范学院学报（自然科学版）Issue(2)：244-247,4.DOI:10.3969/j.issn.1003-0972.2015.02.021
响应面法优化薤白多糖的羧甲基化修饰工艺及抗氧化活性研究
Optimization of Carboxymethylated Modification Technology of Polysaccharides from Allium macrosttemon Bge.and Study on Antioxidative Activity
摘要
Abstract
Total PAM extracted by ethanol precipitation and three hierarchical PAMs extracted by chromatography purified were carboxymethylation modified by chloroacetic acid method.With chloroacetic acid concentration, reaction time and reaction temperature as the independent variables and carboxymethyl substitution degree ( DS ) of modified product as the response value,the modification conditions were optimized by response surface analysis.The antioxidative activity of modified product was tested by H2 O2/Fe2+system and pyrogallol oxidation.The results showed that the opti-mal modification conditions were 1.3 mol/L of chloroacetic acid, the reaction temperature was 63℃and reaction time was 3.2 hours.Under these conditions,the obtained DS was 0.882.Carboxymethylated modification can enhance the antioxidative activity of PAM.关键词
薤白多糖/羧甲基化修饰/响应面法优化/抗氧化活性Key words
Allium macrosttemon Bge.polysaccharides( PAM)/carboxymethylated modification/response surface methodology( RSM)/antioxidative activity分类
轻工纺织引用本文复制引用
夏新奎,豆成林..响应面法优化薤白多糖的羧甲基化修饰工艺及抗氧化活性研究[J].信阳师范学院学报（自然科学版）,2015,(2):244-247,4.基金项目
河南省科技攻关计划项目 （）
