新医学Issue(6)：368-372,5.DOI:10.3969/g.issn.0253-9802.2014.06.005
两种人胚胎干细胞体外诱导C D34＋造血前体细胞方法的比较
Comparison of two methods to generate CD34 +hematopoietic progenitor cells from human embryonic ;stem cells
摘要
Abstract
Objective To compare the efficiencies of two major approaches which have been devel-oped to induce CD34 +hematopoietic progenitor cells (HPCs). They include the coculture of human embryonic stem cells (hESCs)with OP9 stromal cell line and two-dimensional culture system. Methods Induction of hematopoietic differentiation was conducted by coculture of hESCs with OP9 bone marrow stromal cells or through two-dimensional culture system. The efficiencies of CD34 +HPCs differentiation were analyzed by flow cytometry. Results The proportion of CD34 +HPCs developed from hESCs with OP9 coculture system on day 9 was about (19.7 ±7.9)%,while the proportion achieved through two-dimensional culture system on day 6 was about (5.8 ±1.8)% (P<0.05 ). Conclusion Both methods can induce HPCs from hESCs.Two-di-mensional culture system require less time,but the efficiency of hESCs with OP9 coculture system is signifi-cantly higher and will be be more beneficial to the follow-up studies.关键词
人胚胎干细胞/造血前体细胞/体外分化/OP9共培养/二维单层诱导Key words
Human embryonic stem cells/Hematopoietic progenitor cells/In vitro differentiation/OP9 coculture/Two-dimensional monolayer differentiation引用本文复制引用
董晓雅,潘光锦,刘加军..两种人胚胎干细胞体外诱导C D34＋造血前体细胞方法的比较[J].新医学,2014,(6):368-372,5.基金项目
中国医学科学院广州健康研究院开放课题 （）
