新医学Issue(6)：390-393,4.DOI:10.3969/g.issn.0253-9802.2014.06.010
人羊水特异蛋白质组学的探讨研究
Tentative research on the human amniotic fluid proteomics
摘要
Abstract
Objective To explore the specific expression of proteomics in amniotic fluid (AF ).Methods Three samples were collected from normal AF by amniocentesis and peripheral venous blood sam-ples were collected simultaneously from which the serum was isolated. Proteins were extracted from both sam-ples and analyzed by two-dimensional gel electrophoresis (2-DE). The protein blots expressed in normal AF twice as high as that in serum were identified by matrix-assisted laser desorption ionizafion time-of-flight mass spectrometry.Results At pH 4-7 and within a molecular mass of 10-55 kDa,(613 ±29)protein blots were i-dentified in normal AF and (785 ±64)in serum. Approximately 50 protein blots were expressed in normal AF rather than serum. Roughly 50 blots were expressed in normal AF twice as high as that in serum. Conclusion Specific protein expression was observed in normal AF,which probably plays a vital role in pregnancy-related physiology and pathology.关键词
羊水/蛋白质组/双向凝胶电泳/基质辅助激光解吸/离子化飞行时间质谱Key words
Amniotic fluid/Proteomics/Two-dimensional gel electrophoresis/Matrix-assisted laser desorption ionization time-of-flight mass spectrometry引用本文复制引用
朱斌,陈芳,侯常,黎丽红,黄素华..人羊水特异蛋白质组学的探讨研究[J].新医学,2014,(6):390-393,4.基金项目
广东省科技厅社会发展领域科技计划项目 （）
